Recombinant Anti-Kallikrein 14 antibody [EPR7209] - BSA and Azide free (ab248246)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7209] to Kallikrein 14 - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Kallikrein 14 antibody [EPR7209] - BSA and Azide free
See all Kallikrein 14 primary antibodies -
Description
Rabbit monoclonal [EPR7209] to Kallikrein 14 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248246 is the carrier-free version of ab128957.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 1.51 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7209 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 29, 31 kDa (predicted molecular weight: 29 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 29, 31 kDa (predicted molecular weight: 29 kDa). |
Target
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Function
Serine-type endopeptidase with a dual trypsin-like and chymotrypsin-like substrate specificity. May activate/inactivate the proteinase-activated receptors F2R, F2RL1 and F2RL3 and other kallikreins including KLK1, KLK3, KLK5 and KLK11. May function in seminal clot liquefaction through direct cleavage of the semenogelin SEMG1 and SEMG2 and activation of KLK3. May function through desmoglein DSG1 cleavage in epidermal desquamation a process by which the most superficial corneocytes are shed from the skin surface. May be involved in several aspects of tumor progression including growth, invasion and angiogenesis. -
Tissue specificity
Highly expressed in CNS, bone marrow and fetal liver. Also expressed in breast, thyroid, kidney, colon, pancreas, spleen, prostate, uterus, small intestine, placenta and skeletal muscle. Among 40 tissues tested, the highest expression is detected in skin followed by breast and prostate (at protein level). Expressed in stratum corneum by sweat ducts and sweat glands and detected in sweat (at protein level). -
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsProteolytic cleavage of the activation peptide produces the active enzyme. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 43847 Human
- Entrez Gene: 317653 Mouse
- Entrez Gene: 308562 Rat
- Omim: 606135 Human
- SwissProt: Q9P0G3 Human
- SwissProt: Q8CGR5 Mouse
- Unigene: 283925 Human
- Unigene: 247377 Mouse
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Alternative names
- hK14 antibody
- Kallikrein 14 antibody
- Kallikrein like protein 6 antibody
see all
Images
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All lanes : Anti-Kallikrein 14 antibody [EPR7209] (ab128957) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : Fetal liver lysate
Lane 3 : Fetal kidney lysate
Lane 4 : HACAT cell lysate
Lane 5 : Prostate cancer lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 29 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?This data was developed using ab128957, the same antibody clone in a different buffer formulation.
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This data was developed using ab128957, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab248246 has not yet been referenced specifically in any publications.