Anti-Kallikrein 4 antibody (ab181402)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Kallikrein 4 antibody
See all Kallikrein 4 primary antibodies -
Description
Rabbit polyclonal to Kallikrein 4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Kallikrein 4 (internal sequence). Peptide corresponds to 15 amino acids near the center. (NP_004908)
Database link: Q9Y5K2 -
Positive control
- Human kidney tissue and tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab181402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 27 kDa). | |
| ICC/IF | Use a concentration of 20 µg/ml. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Expressed in prostate. -
Involvement in disease
Defects in KLK4 are the cause of amelogenesis imperfecta hypomaturation type 2A1 (AI2A1) [MIM:204700]. AI2A1 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel. -
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 9622 Human
- Omim: 603767 Human
- SwissProt: Q9Y5K2 Human
- Unigene: 218366 Human
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Alternative names
- 7S nerve growth factor alpha chain antibody
- AI2A1 antibody
- Alpha-NGF antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 4 antibody (ab181402)Immunohistochemical analysis of paraffin-embedded Human kidney tissue, labeling Kallikrein 4 using ab181402 at 5 μg/mL.
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Western blot - Anti-Kallikrein 4 antibody (ab181402)Anti-Kallikrein 4 antibody (ab181402) at 1 µg/ml + Human kidney tissue lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Kallikrein 4 antibody (ab181402)Immunofluorescence analysis of Human kidney tissue, labeling Kallikrein 4 using ab181402 at 20 μg/mL.
Protocols
Datasheets and documents
References
ab181402 has not yet been referenced specifically in any publications.
