Anti-Kallikrein 4 antibody (ab181402)
Key features and details
- Rabbit polyclonal to Kallikrein 4
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Kallikrein 4 antibody
See all Kallikrein 4 primary antibodies -
Description
Rabbit polyclonal to Kallikrein 4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human Kallikrein 4 (internal sequence). Peptide corresponds to 15 amino acids near the center. (NP_004908)
Database link: Q9Y5K2 -
Positive control
- Human kidney tissue and tissue lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab181402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 27 kDa). | |
| ICC/IF | Use a concentration of 20 µg/ml. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Expressed in prostate. -
Involvement in disease
Defects in KLK4 are the cause of amelogenesis imperfecta hypomaturation type 2A1 (AI2A1) [MIM:204700]. AI2A1 is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions. The teeth have a shiny agar jelly appearance and the enamel is softer than normal. Brown pigment is present in middle layers of enamel. -
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 9622 Human
- Omim: 603767 Human
- SwissProt: Q9Y5K2 Human
- Unigene: 218366 Human
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Alternative names
- 7S nerve growth factor alpha chain antibody
- AI2A1 antibody
- Alpha-NGF antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 4 antibody (ab181402)Immunohistochemical analysis of paraffin-embedded Human kidney tissue, labeling Kallikrein 4 using ab181402 at 5 μg/mL.
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Western blot - Anti-Kallikrein 4 antibody (ab181402)Anti-Kallikrein 4 antibody (ab181402) at 1 µg/ml + Human kidney tissue lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 27 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Kallikrein 4 antibody (ab181402)Immunofluorescence analysis of Human kidney tissue, labeling Kallikrein 4 using ab181402 at 20 μg/mL.
Protocols
Datasheets and documents
References (1)
ab181402 has been referenced in 1 publication.
- Cheng H et al. Circular RNA Circ_0025033 Promotes the Evolvement of Ovarian Cancer Through the Regulation of miR-330-5p/KLK4 Axis. Cancer Manag Res 12:2753-2765 (2020). PubMed: 32425594
