• Product name

    Anti-Kallikrein 5 antibody
    See all Kallikrein 5 primary antibodies
  • Description

    Rabbit polyclonal to Kallikrein 5
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 280-293 of Human KLK5.



Our Abpromise guarantee covers the use of ab7283 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 32 kDa.
IHC-P Use a concentration of 5 µg/ml.


  • Function

    May be involved in desquamation.
  • Tissue specificity

    Expressed in skin, breast, brain and testis. Expressed at the stratum granulosum of palmar skin.
  • Sequence similarities

    Belongs to the peptidase S1 family. Kallikrein subfamily.
    Contains 1 peptidase S1 domain.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • HK5 antibody
    • Kallikrein 5 antibody
    • Kallikrein L2 antibody
    • Kallikrein like 2 antibody
    • Kallikrein like protein 2 antibody
    • Kallikrein related peptidase 5 antibody
    • Kallikrein-5 antibody
    • Kallikrein-like protein 2 antibody
    • Kallikrein5 antibody
    • KLK 5 antibody
    • KLK L2 antibody
    • KLK-L2 antibody
    • KLK5 antibody
    • KLK5_HUMAN antibody
    • KLKL 2 antibody
    • KLKL2 antibody
    • SCTE antibody
    • Stratum corneum tryptic enzyme antibody
    • UNQ570/PRO1132 antibody
    see all


  • IHC image of ab7283 staining Kallikrein 5 in Human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7283, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ICC/IF image of ab7283 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7283, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Furio L  et al. Transgenic kallikrein 5 mice reproduce major cutaneous and systemic hallmarks of Netherton syndrome. J Exp Med 211:499-513 (2014). Read more (PubMed: 24534191) »
  • Bonnart C  et al. Elastase 2 is expressed in human and mouse epidermis and impairs skin barrier function in Netherton syndrome through filaggrin and lipid misprocessing. J Clin Invest 120:871-82 (2010). IHC-P ; Human . Read more (PubMed: 20179351) »
See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A


L’immunogène d’ab7283 correspond aux acides amines 280-293 de la protéine kallikrein 5 humaine. Ces acides aminés sont présents chez la forme active de la protéine.

L’immunogène d’ab96710 se trouve dans les acides aminés 30-253 de la protéine kallikrein 7 qui est la forme active de la protéine.

Je peux donc vous confirmer que ces deux anticorps reconnaissent à la fois les formes actives et non actives de ces protéines.

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Please find attached the Material Safety Datasheet for some of the products requested. Those products not containing any hazardous products do not require MSDS.
If by any chance you required these datasheets for legal requirements, please do not hesitate to let me know, as we can still prepare it for you.

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Thank you for your enquiry. The antibody ab7283 has been tested in standard reducing conditions in western blotting and has not been tested in IHC-P. It may work in IHC-P and should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will offer you 50 Abpoints which can be redeemed on a number of rewards (a further 100 Abpoints will be offered for an image).

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