Anti-Kallikrein 5 antibody (ab7283)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-Kallikrein 5 antibody
See all Kallikrein 5 primary antibodies -
DescriptionRabbit polyclonal to Kallikrein 5
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide, corresponding to amino acids 280-293 of Human KLK5.
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Positive control
- MCF-7 and ZR75-1 cell lysates (see review)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPreservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab7283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | 1/500 - 1/1000. Detects a band of approximately 32 kDa. | |
| IHC-P | Use a concentration of 5 µg/ml. |
Target
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FunctionMay be involved in desquamation.
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Tissue specificityExpressed in skin, breast, brain and testis. Expressed at the stratum granulosum of palmar skin.
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Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 25818 Human
- Omim: 605643 Human
- SwissProt: Q9Y337 Human
- Unigene: 50915 Human
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Alternative names
- HK5 antibody
- Kallikrein 5 antibody
- Kallikrein L2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kallikrein 5 antibody (ab7283)IHC image of ab7283 staining Kallikrein 5 in Human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7283, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunocytochemistry/ Immunofluorescence - Anti-Kallikrein 5 antibody (ab7283)ICC/IF image of ab7283 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7283, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
References
This product has been referenced in:
- Furio L et al. Transgenic kallikrein 5 mice reproduce major cutaneous and systemic hallmarks of Netherton syndrome. J Exp Med 211:499-513 (2014). Read more (PubMed: 24534191) »
- Bonnart C et al. Elastase 2 is expressed in human and mouse epidermis and impairs skin barrier function in Netherton syndrome through filaggrin and lipid misprocessing. J Clin Invest 120:871-82 (2010). IHC-P ; Human . Read more (PubMed: 20179351) »
