Key features and details
- Rabbit polyclonal to Kallikrein 8/KLK8
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Kallikrein 8/KLK8 antibody
See all Kallikrein 8/KLK8 primary antibodies
DescriptionRabbit polyclonal to Kallikrein 8/KLK8
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant full length protein (His-T7-tag) corresponding to Human Kallikrein 8/KLK8 aa 29-260. (Expressed in E.coli).
QEDKVLGGHECQPHSQPWQAALFQGQQLLCGGVLVGGNWVLTAAHCKKPK YTVRLGDHSLQNKDGPEQEIPVVQSIPHPCYNSSDVEDHNHDLMLLQLRD QASLGSKVKPISLADHCTQPGQKCTVSGWGTVTSPRENFPDTLNCAEVKI FPQKKCEDAYPGQITDGMVCAGSSKGADTCQGDSGGPLVCDGALQGITSW GSDPCGRSDKPGVYTNICRYLDWIKKIIGSKG
Database link: O60259
- WB: Mouse ear lysate; Recombinant human Kallikrein 8/KLK8 protein. IHC-P: Human pancreas tissue.
This product was previously labelled as Kallikrein 8
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab232839 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232839 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 28 kDa.|
FunctionSerine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury.
Tissue specificityIsoform 1 is predominantly expressed in the pancreas. Isoform 2 is expressed in adult brain and hippocampus. Isoform 1 and isoform 2 are found in fetal brain and placenta. Detected in salivary gland, uterus, thymus, breast, testis and kidney but not in spleen, liver, lung or normal ovarian tissue. Displays an 11.5-fold increase in Alzheimer disease hippocampus compared to controls and is overexpressed in some ovarian carcinomas. Expressed at low levels in normal skin while high levels are found in psoriasis vulgaris, seborrheic keratosis, lichen planus and squamous cell carcinoma skin samples. Expressed in the keratinocytes.
Sequence similaritiesBelongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain.
Cellular localizationSecreted. Cytoplasm. Shows a cytoplasmic distribution in the keratinocytes.
- Information by UniProt
- Brain serine protease 1 antibody
- BSP1 antibody
- hK8 antibody
Formalin-fixed, paraffin-embedded human pancreas tissue stained for Kallikrein 8/KLK8 using ab232839 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-Kallikrein 8/KLK8 antibody (ab232839) at 1 µg/ml + Mouse ear lysate
HRP-Linked Guinea pig anti-rabbit at 1/1000 dilution
Predicted band size: 28 kDa
Anti-Kallikrein 8/KLK8 antibody (ab232839) at 2 µg/ml + Recombinant human Kallikrein 8/KLK8 protein
Predicted band size: 28 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232839 has not yet been referenced specifically in any publications.