Recombinant Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] (ab150395)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5752(2)] to Kallikrein 8/KLK8
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)]
See all Kallikrein 8/KLK8 primary antibodies -
Description
Rabbit monoclonal [EPR5752(2)] to Kallikrein 8/KLK8 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Kallikrein 8/KLK8 aa 200-300 (C terminal). The exact sequence is proprietary.
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Positive control
- Jurkat, Human fetal brain, A431, and HeLa whole cell lysate (ab150035). Human endometrial carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5752(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 28 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury. -
Tissue specificity
Isoform 1 is predominantly expressed in the pancreas. Isoform 2 is expressed in adult brain and hippocampus. Isoform 1 and isoform 2 are found in fetal brain and placenta. Detected in salivary gland, uterus, thymus, breast, testis and kidney but not in spleen, liver, lung or normal ovarian tissue. Displays an 11.5-fold increase in Alzheimer disease hippocampus compared to controls and is overexpressed in some ovarian carcinomas. Expressed at low levels in normal skin while high levels are found in psoriasis vulgaris, seborrheic keratosis, lichen planus and squamous cell carcinoma skin samples. Expressed in the keratinocytes. -
Sequence similarities
Belongs to the peptidase S1 family. Kallikrein subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Secreted. Cytoplasm. Shows a cytoplasmic distribution in the keratinocytes. - Information by UniProt
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Database links
- Entrez Gene: 11202 Human
- Omim: 605644 Human
- SwissProt: O60259 Human
- Unigene: 104570 Human
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Alternative names
- Brain serine protease 1 antibody
- BSP1 antibody
- hK8 antibody
see all
Images
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All lanes : Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] (ab150395) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Human fetal brain cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit
Predicted band size: 28 kDa -
Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labelling Kallikrein 8/KLK8 with ab150395 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab150395 has been referenced in 6 publications.
- Du JK et al. A novel role of kallikrein-related peptidase 8 in the pathogenesis of diabetic cardiac fibrosis. Theranostics 11:4207-4231 (2021). PubMed: 33754057
- Hua Q et al. Upregulation of KLK8 Predicts Poor Prognosis in Pancreatic Cancer. Front Oncol 11:624837 (2021). PubMed: 34395235
- Hua Q et al. KLK8 promotes the proliferation and metastasis of colorectal cancer via the activation of EMT associated with PAR1. Cell Death Dis 12:860 (2021). PubMed: 34552064
- Lim SC et al. Extracellular acidity-induced expression of Kallikrein-related peptidases 7 and 8 is involved in increased invasiveness of gastric cancer cells. Oncol Rep 43:1705-1713 (2020). PubMed: 32323843
- Aseervatham J & Ogbureke KUE Effects of DSPP and MMP20 Silencing on Adhesion, Metastasis, Angiogenesis, and Epithelial-Mesenchymal Transition Proteins in Oral Squamous Cell Carcinoma Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32630820
- Lei S et al. Expression and clinical significance of KLK5-8 in endometrial cancer. Am J Transl Res 11:4180-4191 (2019). PubMed: 31396327