Overview

  • Product name

    Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)]
    See all Kallikrein 8/KLK8 primary antibodies
  • Description

    Rabbit monoclonal [EPR5752(2)] to Kallikrein 8/KLK8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Kallikrein 8/KLK8 aa 200-300 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Jurkat, Human fetal brain, A431, and HeLa whole cell lysate (ab150035). Human endometrial carcinoma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as Kallikrein 8

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab150395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury.
    • Tissue specificity

      Isoform 1 is predominantly expressed in the pancreas. Isoform 2 is expressed in adult brain and hippocampus. Isoform 1 and isoform 2 are found in fetal brain and placenta. Detected in salivary gland, uterus, thymus, breast, testis and kidney but not in spleen, liver, lung or normal ovarian tissue. Displays an 11.5-fold increase in Alzheimer disease hippocampus compared to controls and is overexpressed in some ovarian carcinomas. Expressed at low levels in normal skin while high levels are found in psoriasis vulgaris, seborrheic keratosis, lichen planus and squamous cell carcinoma skin samples. Expressed in the keratinocytes.
    • Sequence similarities

      Belongs to the peptidase S1 family. Kallikrein subfamily.
      Contains 1 peptidase S1 domain.
    • Cellular localization

      Secreted. Cytoplasm. Shows a cytoplasmic distribution in the keratinocytes.
    • Information by UniProt
    • Database links

    • Alternative names

      • Brain serine protease 1 antibody
      • BSP1 antibody
      • hK8 antibody
      • HNP antibody
      • HPN antibody
      • Kallikrein 8 (neuropsin/ovasin) antibody
      • Kallikrein related peptidase 8 antibody
      • Kallikrein-8 antibody
      • Kallikrein8 antibody
      • KLK 8 antibody
      • Klk8 antibody
      • KLK8 protein type 1 antibody
      • KLK8 protein type 2 antibody
      • KLK8_HUMAN antibody
      • Neuropsin antibody
      • neuropsin type 1 antibody
      • Neuropsin type 1, included antibody
      • Neuropsin type 2 antibody
      • Neuropsin type 2, included antibody
      • Neuropsin, mouse, homolog of antibody
      • NP antibody
      • NRPN antibody
      • Ovasin antibody
      • PRO322 antibody
      • Protease serine 19 antibody
      • PRSS 19 antibody
      • PRSS19 antibody
      • Serine protease 19 antibody
      • Serine protease kallikrein antibody
      • Serine protease kallikrein/ovasin/neuropsin antibody
      • Serine protease TADG 14 antibody
      • Serine protease TADG-14 antibody
      • Serine protease TADG14 antibody
      • TADG 14 antibody
      • TADG14 antibody
      • Tumor associated differentially expressed gene 14 antibody
      • Tumor associated differentially expressed gene 14 protein antibody
      • Tumor-associated differentially expressed gene 14 protein antibody
      • UNQ283 antibody
      see all

    Images

    • All lanes : Anti-Kallikrein 8/KLK8 antibody [EPR5752(2)] (ab150395) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Human fetal brain cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit

      Predicted band size: 28 kDa

    • Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labelling Kallikrein 8/KLK8 with ab150395 at 1/50 dilution.

    References

    ab150395 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab150395.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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