Overview

  • Product name

    Anti-Kallistatin/PI-4 antibody
    See all Kallistatin/PI-4 primary antibodies
  • Description

    Rabbit polyclonal to Kallistatin/PI-4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Kallistatin/PI-4 aa 115-354 (internal sequence).
    Database link: P29622

  • Positive control

    • HCT116 whole cell lysate; HCT116 cells; Human colon carcinoma tissue
  • General notes

    Previously labelled as Kallistatin. 

Properties

Applications

Our Abpromise guarantee covers the use of ab154597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 49 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol, using 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein.
  • Tissue specificity

    Expressed by the liver and secreted in plasma.
  • Sequence similarities

    Belongs to the serpin family.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • KAIN_HUMAN antibody
    • KAL antibody
    • Kallikrein binding protein antibody
    • Kallikrein inhibitor antibody
    • Kallistatin antibody
    • KBP antibody
    • KLST antibody
    • KST antibody
    • MGC108582 antibody
    • Peptidase inhibitor 4 antibody
    • PI-4 antibody
    • PI4 antibody
    • Protease inhibitor 4 (kallistatin) antibody
    • Protease inhibitor 4 antibody
    • Serine (or cysteine) proteinase inhibitor, clade A (alpha 1 antiproteinase, antitrypsin), member 4 antibody
    • Serpin A4 antibody
    • Serpin peptidase inhibitor clade A member 4 antibody
    • Serpin peptidase inhibitor, clade A (alpha 1 antiproteinase, antitrypsin), member 4 antibody
    • SERPINA4 antibody
    • Tissue kallistatin inhibitor antibody
    see all

Images

  • Immunofluorescent analysis of methanol-fixed HCT116 cells labeling Kallistatin/PI-4 with ab154597 at 1/500 dilution.
    The image in the lower panel was co-stained with Hoechst 33342.

  • Anti-Kallistatin/PI-4 antibody (ab154597) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size: 49 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling Kallistatin/PI-4 with ab154597 at 1/250 dilution.

References

ab154597 has not yet been referenced specifically in any publications.

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