Overview

  • Product name
    Anti-KALRN/DUET antibody
  • Description
    Goat polyclonal to KALRN/DUET
  • Host species
    Goat
  • Specificity
    This antibody is expected to recognize isoform 2 (NP_003938.1).
  • Tested applications
    Suitable for: WB, ELISA, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human KALRN/DUET aa 1650-1663 (C terminal).
    Sequence:

    C-RWHLGPGDPFSTYV

  • Positive control
    • Mouse Brain lysate.
  • General notes

     This product was previously labelled as KALRN

     

Properties

Applications

Our Abpromise guarantee covers the use of ab52012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 192 kDa).
ELISA Use at an assay dependent concentration. Peptide ELISA: antibody detection limit dilution 1:8000.
IHC-Fr 1/200.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Promotes the exchange of GDP by GTP. Activates specific Rho GTPase family members, thereby inducing various signaling mechanisms that regulate neuronal shape, growth, and plasticity, through their effects on the actin cytoskeleton. Induces lamellipodia independent of its GEF activity.
  • Tissue specificity
    Isoform 2 is brain specific. Highly expressed in cerebral cortex, putamen, amygdala, hippocampus and caudate nucleus. Weakly expressed in brain stem and cerebellum. Isoform 4 is expressed in skeletal muscle.
  • Involvement in disease
    Genetic variation in KALRN is associated with susceptibility to coronary heart disease type 5 (CHDS5) [MIM:608901]. CHD is the leading cause of death and disability worldwide. CHD is multifactorial disease with a strong genetic component. Classic epidemiologic studies have revealed many risk factors for CHD, including age, sex, hypertension, dyslipidemia, diabetes mellitus, smoking, and physical inactivity.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 CRAL-TRIO domain.
    Contains 2 DH (DBL-homology) domains.
    Contains 1 fibronectin type-III domain.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 2 PH domains.
    Contains 1 protein kinase domain.
    Contains 2 SH3 domains.
    Contains 5 spectrin repeats.
  • Domain
    The two GEF domains catalyze nucleotide exchange for RAC1 and RhoA which are bound by DH1 and DH2 respectively. The two GEF domains appear to play differing roles in neuronal development and axonal outgrowth. SH3 1 binds to the first GEF domain inhibiting GEF activity only when in the presence of a PXXP peptide, suggesting that the SH3 domain/peptide interaction mediates binding to GEF1. CRK1 SH3 domain binds to and inhibits GEF1 activity.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton. Associated with the cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Duo antibody
    • FLJ16443 antibody
    • HAPIP antibody
    • Huntingtin associated protein interacting protein (duo) antibody
    • Huntingtin-associated protein-interacting protein antibody
    • Kalirin (isoform 2) antibody
    • Kalirin antibody
    • KALRN antibody
    • KALRN_HUMAN antibody
    • Protein Duo antibody
    • RhoGEF kinase antibody
    • Serine/threonine kinase with Dbl and pleckstrin homology domains antibody
    • Serine/threonine-protein kinase with Dbl- and pleckstrin homology domain antibody
    • TRAD antibody
    see all

Images

  • ab52012 staining KALRN/DUET in adult brain tissue by Immunohistochemistry (paraffin embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step performed using citrate buffer. Tissues were then blocked with 15% serum for 45 minutes at 20°C followed by incubation with the primary antibody, at a 1/250 dilution, for 24 hours at 4°C. A Biotin-conjugated rabbit anti-goat IgG was used as secondary antibody at a 1/250 dilution.Upper image: Un-treated.Lower image: Treated with peptide to KALRN/DUET. Counterstained with Hämalaun.

    See Abreview

  • All lanes : Anti-KALRN/DUET antibody (ab52012) at 1/500 dilution

    Lane 1 : Whole tissue lysate prepared from mouse brain, treated with peptide.
    Lane 2 : Whole cell lysate prepared from mouse brain cells over-expressing KALRN/DUET (with FLAG-tag) treated with peptide.
    Lane 3 : Whole cell lysate prepared from mouse brain cells over-expressing KALRN/DUET, with FLAG-tag, detected with anti-FLAG antibody.
    Lane 4 : Whole tissue lysate prepared from mouse brain
    Lane 5 : Whole cell lysate prepared from mouse brain cells over-expressing KALRN/DUET, with FLAG-tag.

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Donkey anti-goat IgG conjugated to HRP at 1/10000 dilution

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 192 kDa
    Observed band size: 192 kDa


    Exposure time: 1 minute


    8% gel run under denaturing conditions.

    See Abreview

  • Anti-KALRN/DUET antibody (ab52012) at 0.5 µg/ml + Mouse Brain lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 192 kDa



    Primary incubation was 1 hour.

References

This product has been referenced in:
  • Parato J  et al. a4ßd GABAA Receptors Trigger Synaptic Pruning and Reduce Dendritic Length of Female Mouse CA3 Hippocampal Pyramidal Cells at Puberty. Neuroscience 398:23-36 (2019). Read more (PubMed: 30496825) »
  • Afroz S  et al. Synaptic pruning in the female hippocampus is triggered at puberty by extrasynaptic GABAA receptors on dendritic spines. Elife 5:N/A (2016). ICC . Read more (PubMed: 27136678) »
See all 4 Publications for this product

Customer reviews and Q&As

1-10 of 14 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Whole brain sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - 0.05% Tween-20
Specification
Whole brain sections
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 02 2017

Application
Western blot
Sample
Mouse Tissue lysate - whole (Whole brain tissue lysate)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (3-8% Bis-Tris)
Loading amount
20 µg
Specification
Whole brain tissue lysate
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

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Verified customer

Submitted Jun 02 2017

Application
Western blot
Sample
Cow Cell lysate - whole cell (Lung)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Lung
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

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Verified customer

Submitted Apr 11 2017

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Brain)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12)
Loading amount
30 µg
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 11 2017

Application
Western blot
Sample
African green monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 11 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 11 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (kidney)
Permeabilization
Yes - 0.2% triton x100
Specification
kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 24 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (kidney)
Permeabilization
Yes - 0.2% triton x100
Specification
kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 24 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.3% triton x100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 24 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (mouse spinal cord, 20 micron)
Permeabilization
Yes - Triton X-100
Specification
mouse spinal cord, 20 micron
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Apr 11 2014

1-10 of 14 Abreviews or Q&A

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