Overview

  • Product name
    Anti-kan antibody [4B4D1]
    See all kan primary antibodies
  • Description
    Mouse monoclonal [4B4D1] to kan
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant fragment
    Predicted to work with: Escherichia coli
  • Immunogen

    Recombinant full length protein corresponding to kan.

  • General notes

    Previously labelled as Neomycin Phosphotransferase 2. 

Properties

Applications

Our Abpromise guarantee covers the use of ab60018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa).
ELISA 1/10000.

Target

  • Relevance
    Neomycin Phosphotransferase 2 (kan) confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.
  • Database links
    • Alternative names
      • Aminoglycoside 3' phosphotransferase antibody
      • APH(3') II antibody
      • APH(3')II antibody
      • aphA antibody
      • EC 2.7.1.95 antibody
      • Kan antibody
      • Kanamycin kinase type II antibody
      • Kanamycin kinase, type VIII antibody
      • kanamycin resistance gene antibody
      • Neo antibody
      • Neomycin kanamycin phosphotransferase type II antibody
      • Neomycin kanamycin phosphotransferase type VIII antibody
      • Neomycin Phosphotransferase 2 antibody
      • Neomycin phosphotransferase II antibody
      • Neomycine phosphotransferase antibody
      • NPT II antibody
      • NPTII antibody
      see all

    Images

    References

    This product has been referenced in:
    • Kallehauge TB  et al. Ribosome profiling-guided depletion of an mRNA increases cell growth rate and protein secretion. Sci Rep 7:40388 (2017). Read more (PubMed: 28091612) »
    • Bentham MJ  et al. NS2 is dispensable for efficient assembly of hepatitis C virus-like particles in a bipartite trans-encapsidation system. J Gen Virol 95:2427-41 (2014). Read more (PubMed: 25024280) »
    See all 10 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Application
    Western blot
    Sample
    Arabidopsis thaliana Tissue lysate - whole (seedlings)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    100 µg
    Treatment
    protease inhibitor
    Specification
    seedlings
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

    Nico Dissmeyer

    Verified customer

    Submitted Aug 03 2015

    Answer

    I am very pleased to hear you would like to accept our offer and test ab60018 in ICC-IF

    DISCOUNT CODE: #####
    Expiration date: ######

    This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forICC-IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    Read More

    Answer

    Thank you for your enquiry.

    I can confirm that to our knowledge, ab33595 and ab60018 have not yet been tested in ICC (immunocytochemistry staining of cell culture), IHC-Frozen sections (IHC-Fr) or IHC-Paraffin tissue sections (IHC-P).

    Therefore, I can offer a discount off a future purchase if you buy either ab33595 or ab60018 now, test it inICC-IF, IHC-Fr or IHC-P and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 freeprimary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and which antibodythe customer would like to test. Please also confirm which application (ICC, IHC-Fr or IHC-P). I will then send a discount code. This code must be issued before purchasing the antibody so please wait for my reply before ordering.

    2. Purchasethe antibodyeither by phone, fax, or online (www.abcam.com).

    3. Test it in ICC, IHC-Fr or IHC-P.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibody ordered and the discount code is valid for 4 months after issue.

    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifthe antibodyturns out to be unsuitable for ICC, IHC-Fr or IHC-P, you will still receive the discount on your next purchase after your Abreview has been submitted.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up