Overview

  • Product name
    Anti-kan antibody [4B4D1]
    See all kan primary antibodies
  • Description
    Mouse monoclonal [4B4D1] to kan
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant fragment
    Predicted to work with: Escherichia coli
  • Immunogen

    Recombinant full length protein corresponding to kan.

  • General notes

    Previously labelled as Neomycin Phosphotransferase 2. 

Properties

Applications

Our Abpromise guarantee covers the use of ab60018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Detects a band of approximately 30 kDa (predicted molecular weight: 29 kDa).
ELISA 1/10000.

Target

  • Relevance
    Neomycin Phosphotransferase 2 (kan) confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.
  • Database links
    • Alternative names
      • Aminoglycoside 3' phosphotransferase antibody
      • APH(3') II antibody
      • APH(3')II antibody
      • aphA antibody
      • EC 2.7.1.95 antibody
      • Kan antibody
      • Kanamycin kinase type II antibody
      • Kanamycin kinase, type VIII antibody
      • kanamycin resistance gene antibody
      • Neo antibody
      • Neomycin kanamycin phosphotransferase type II antibody
      • Neomycin kanamycin phosphotransferase type VIII antibody
      • Neomycin Phosphotransferase 2 antibody
      • Neomycin phosphotransferase II antibody
      • Neomycine phosphotransferase antibody
      • NPT II antibody
      • NPTII antibody
      see all

    Images

    References

    This product has been referenced in:
    • Kallehauge TB  et al. Ribosome profiling-guided depletion of an mRNA increases cell growth rate and protein secretion. Sci Rep 7:40388 (2017). Read more (PubMed: 28091612) »
    • Bentham MJ  et al. NS2 is dispensable for efficient assembly of hepatitis C virus-like particles in a bipartite trans-encapsidation system. J Gen Virol 95:2427-41 (2014). Read more (PubMed: 25024280) »
    See all 10 Publications for this product

    Customer reviews and Q&As

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    Application
    Western blot
    Sample
    Arabidopsis thaliana Tissue lysate - whole (seedlings)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    100 µg
    Treatment
    protease inhibitor
    Specification
    seedlings
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

    Nico Dissmeyer

    Verified customer

    Submitted Aug 03 2015

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