Product nameAnti-kan antibody
See all kan primary antibodies
DescriptionRabbit polyclonal to kan
Tested applicationsSuitable for: WB, ELISAmore details
Synthetic peptide within kan. The exact sequence is proprietary. Klebsiella pneumoniae.
Database link: P00552
Previously labelled as Neomycin Phosphotransferase 2.
FormLyophilised:Must be reconstituted in 100µL of distilled water.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab182391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 29 kDa.|
|ELISA||Use at an assay dependent concentration.|
RelevanceNeomycin Phosphotransferase 2 (kan) confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.
- Aminoglycoside 3' phosphotransferase antibody
- APH(3') II antibody
- APH(3')II antibody
ab182391 has not yet been referenced specifically in any publications.