Overview

  • Product name

  • Description

    Rabbit polyclonal to kan
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Immunogen

    Synthetic peptide within kan. The exact sequence is proprietary. Klebsiella pneumoniae.
    Database link: P00552

  • General notes

    Previously labelled as Neomycin Phosphotransferase 2. 

Properties

Applications

Our Abpromise guarantee covers the use of ab182391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 29 kDa.
ELISA Use at an assay dependent concentration.

Target

  • Relevance

    Neomycin Phosphotransferase 2 (kan) confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.
  • Alternative names

    • Aminoglycoside 3' phosphotransferase antibody
    • APH(3') II antibody
    • APH(3')II antibody
    • aphA antibody
    • EC 2.7.1.95 antibody
    • Kan antibody
    • Kanamycin kinase type II antibody
    • Kanamycin kinase, type VIII antibody
    • kanamycin resistance gene antibody
    • Neo antibody
    • Neomycin kanamycin phosphotransferase type II antibody
    • Neomycin kanamycin phosphotransferase type VIII antibody
    • Neomycin Phosphotransferase 2 antibody
    • Neomycin phosphotransferase II antibody
    • Neomycine phosphotransferase antibody
    • NPT II antibody
    • NPTII antibody
    see all

References

ab182391 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer



Unfortunately, I can confirm that if you are amplifying the Neo gene from pAcGFP N1 sequence you sent me, there is no homology with the immunogen used to raise the ab49975 antibody, so we would not expect to cross react with the expressed protein. This antibody recognizes the internal sequence amino acids 133-150 of Escherichia coli Neomycin Phosphotransferase 2.

I have double checked your sequence and compared it with other immunogens, and I am happy to confirm you that the Anti-Neomycin Phosphotransferase 2 antibody (ab182391) has a high homology with your sequence (99%), so we expect it to recognize the protein. Further this antibody is covered by our Abpromise guaranteed for WB and ELISA (www.abcam.com/abpromise). See more details of the antibody here:

https://www.abcam.com/index.html?datasheet=182391 (or use the following: https://www.abcam.com/index.html?datasheet=182391).

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