Product nameAnti-kan antibody
See all kan primary antibodies
DescriptionRabbit polyclonal to kan
Tested applicationsSuitable for: ELISA, WB, IHC-Frmore details
Species reactivityReacts with bacteria. Not yet tested in other species.
Full length protein corresponding to kan.
Previously labelled as Neomycin Phosphotransferase 2.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 40% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab33595 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Predicted molecular weight: 32 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
RelevanceNeomycin Phosphotransferase 2 (kan) confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.
- Aminoglycoside 3' phosphotransferase antibody
- APH(3') II antibody
- APH(3')II antibody
This product has been referenced in:
- Byun H et al. A lower isoelectric point increases signal sequence-mediated secretion of recombinant proteins through a bacterial ABC transporter. J Biol Chem 292:19782-19791 (2017). Read more (PubMed: 28972172) »
- Yamaguchi K et al. Regulation of plasma-membrane-associated sialidase NEU3 gene by Sp1/Sp3 transcription factors. Biochem J 430:107-17 (2010). ChIP ; Human . Read more (PubMed: 20518744) »