Key features and details
- Mouse monoclonal [A8B5] to Kappa light chain
- Suitable for: ELISA, IHC-P, IHC-Fr, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Kappa light chain antibody [A8B5]
See all Kappa light chain primary antibodies
DescriptionMouse monoclonal [A8B5] to Kappa light chain
Tested applicationsSuitable for: ELISA, IHC-P, IHC-Fr, Flow Cytmore details
Species reactivityReacts with: Human
Does not react with: Sheep, Rabbit, Goat, Guinea pig, Hamster
The details of the immunogen for this antibody are not available.
- Daudi human cell line.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notesab79558 is purified from hybridoma culture supernatant by protein A affinity chromatography. Purity is > 95% (by SDS-PAGE).
Our Abpromise guarantee covers the use of ab79558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
RelevanceImmunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
Cellular localizationCytoplasmic and Secreted
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
ab79558 detecting Kappa light chain in B lymphocytes by Flow cytometry. B lymphocytes (CD19+) in a dot-plot Lambda PE vs. Kappa FITC. Kappa light chain detected by A8B5 antibody and lambda light chain by 4C2, CD19 by LT19 antibody (ab8331).
ab79558 has been referenced in 3 publications.
- Di Bartolo DL et al. Role of defective Oct-2 and OCA-B expression in immunoglobulin production and Kaposi's sarcoma-associated herpesvirus lytic reactivation in primary effusion lymphoma. J Virol 83:4308-15 (2009). PubMed: 19224997
- Scheeren FA et al. IL-21 is expressed in Hodgkin lymphoma and activates STAT5: evidence that activated STAT5 is required for Hodgkin lymphomagenesis. Blood 111:4706-15 (2008). PubMed: 18296629
- Scheeren FA et al. STAT5 regulates the self-renewal capacity and differentiation of human memory B cells and controls Bcl-6 expression. Nat Immunol 6:303-13 (2005). PubMed: 15711548