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    kappa-light-chain-antibody-ab103827.pdf

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Immunology Immunoglobulins Light Chain Kappa
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Anti-Kappa light chain antibody (ab103827)

  • Datasheet
Submit a review Q&A (1)References (1)

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Abpromise

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Western blot - Anti-Kappa light chain antibody (ab103827)
  • Western blot - Anti-Kappa light chain antibody (ab103827)

Key features and details

  • Rabbit polyclonal to Kappa light chain
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Kappa light chain antibody
    See all Kappa light chain primary antibodies
  • Description

    Rabbit polyclonal to Kappa light chain
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-234 of Human Kappa light chain (AAH66343.1).

  • Positive control

    • Mouse kidney. Transfected 293T cell line.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Immunoglobulins
    • Light Chain
    • Kappa

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab103827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 12 kDa.

Target

  • Relevance

    Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
  • Cellular localization

    Cytoplasmic and Secreted
  • Database links

    • Entrez Gene: 28901 Human
    • Entrez Gene: 3514 Human
    • Entrez Gene: 16071 Mouse
    • Omim: 147200 Human
    • SwissProt: P01601 Human
    • SwissProt: P01834 Human
    • SwissProt: P01837 Mouse
    • Unigene: 449609 Human
    • Unigene: 333124 Mouse
    see all
  • Alternative names

    • HCAK 1 antibody
    • HCAK1 antibody
    • Ig kappa chain C region antibody
    • IGKC antibody
    • IGKCD antibody
    • Immunoglobulin InV antibody
    • Immunoglobulin kappa constant antibody
    • Immunoglobulin kappa constant region antibody
    • Immunoglobulin kappa light chain antibody
    • Immunoglobulin kappa light chain constant region antibody
    • Immunoglobulin KM antibody
    • Kappa 1 immunoglobulin light chain antibody
    • kappa light chain antibody
    • Km antibody
    • MGC111575 antibody
    • MGC62011 antibody
    • MGC72072 antibody
    • MGC88770 antibody
    • MGC88771 antibody
    • MGC88809 antibody
    see all

Images

  • Western blot - Anti-Kappa light chain antibody (ab103827)
    Western blot - Anti-Kappa light chain antibody (ab103827)
    Anti-Kappa light chain antibody (ab103827) at 5 µg/ml + Mouse kidney tissue lysate at 50 µg

    Predicted band size: 12 kDa

  • Western blot - Anti-Kappa light chain antibody (ab103827)
    Western blot - Anti-Kappa light chain antibody (ab103827)
    All lanes : Anti-Kappa light chain antibody (ab103827) at 5 µg/ml

    Lane 1 : Kappa light chain transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 12 kDa

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab103827? Please let us know so that we can cite the reference in this datasheet.

    ab103827 has been referenced in 1 publication.

    • Baldini C  et al. Proteomic analysis of saliva: a unique tool to distinguish primary Sjögren's syndrome from secondary Sjögren's syndrome and other sicca syndromes. Arthritis Res Ther 13:R194 (2011). WB . PubMed: 22117835

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Dear xxxxx,


    Thanks for your email.

    In mice, 95% of the light chains are κ and only 5% are λ, perhaps you can quote both κ light chain and λ light chain.

    Thanks and best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Oct 10 2012

    Answer

    Thank you for your reply.

    I have had a look at the antibodies we have available form our catalogue and have come up with the following for you to consider:

    against kappa light chain:

    Rat monoclonal ab11662

    Rat monoclonal ab99614

    Rabbit polyclonal ab103827

    against kappa light chain, HRP conjugated:

    Goat polyclonal ab46527

    Rat monoclonal ab99617

    against lambda light chain:

    Rat monoclonal ab99622

    against lambda light chain HRP conjugated

    Rat monoclonal ab99625

    I hope this information has been of help. If you have any further questions, please do not hesitate to contact me again.

    Read More

    Abcam Scientific Support

    Answered on Oct 10 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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