Key features and details
- Rabbit polyclonal to Kappa light chain
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Kappa light chain antibody
See all Kappa light chain primary antibodies
DescriptionRabbit polyclonal to Kappa light chain
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Full length protein, corresponding to amino acids 1-234 of Human Kappa light chain (AAH66343.1).
- Mouse kidney. Transfected 293T cell line.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab103827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 12 kDa.|
RelevanceImmunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
Cellular localizationCytoplasmic and Secreted
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
Anti-Kappa light chain antibody (ab103827) at 5 µg/ml + Mouse kidney tissue lysate at 50 µg
Predicted band size: 12 kDa
All lanes : Anti-Kappa light chain antibody (ab103827) at 5 µg/ml
Lane 1 : Kappa light chain transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 12 kDa
ab103827 has been referenced in 1 publication.
- Baldini C et al. Proteomic analysis of saliva: a unique tool to distinguish primary Sjögren's syndrome from secondary Sjögren's syndrome and other sicca syndromes. Arthritis Res Ther 13:R194 (2011). WB . PubMed: 22117835