Overview

  • Product name

    Anti-Kappa light chain antibody
    See all Kappa light chain primary antibodies
  • Description

    Rabbit polyclonal to Kappa light chain
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    corresponding to Human Kappa light chain.
    (Peptide available as ab216266)

  • Positive control

    • Human tonsil and Human plasma lysates, Human tonsil tissue
  • General notes

    Produced under U.S. Patent No. 5,675,063.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 25 kDa (predicted molecular weight: 12 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Relevance

      Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
    • Cellular localization

      Cytoplasmic and Secreted
    • Database links

    • Alternative names

      • HCAK 1 antibody
      • HCAK1 antibody
      • Ig kappa chain C region antibody
      • IGKC antibody
      • IGKCD antibody
      • Immunoglobulin InV antibody
      • Immunoglobulin kappa constant antibody
      • Immunoglobulin kappa constant region antibody
      • Immunoglobulin kappa light chain antibody
      • Immunoglobulin kappa light chain constant region antibody
      • Immunoglobulin KM antibody
      • Kappa 1 immunoglobulin light chain antibody
      • kappa light chain antibody
      • Km antibody
      • MGC111575 antibody
      • MGC62011 antibody
      • MGC72072 antibody
      • MGC88770 antibody
      • MGC88771 antibody
      • MGC88809 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling Kappa light chain with ab134083 at 1/250.
    • All lanes : Anti-Kappa light chain antibody (ab134083) at 1/10000 dilution

      Lane 1 : Human tonsil lysate
      Lane 2 : Human plasma lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-rabbit at 1/2000 dilution

      Predicted band size: 12 kDa
      Observed band size: 25 kDa
      why is the actual band size different from the predicted?

    • All lanes : Anti-Kappa light chain antibody (ab134083) at 1/10000 dilution

      Lane 1 : Kappa light chain purified protein
      Lane 2 : Lambda light chain purified protein

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-rabbit at 1/2000 dilution

      Predicted band size: 12 kDa
      Observed band size: 25 kDa why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Kim M  et al. Applications of the immunoglobulin Cw fragment (IgCw) composed of the constant regions of heavy and light (CH and CL) chains. Biochem Biophys Res Commun 512:571-576 (2019). Read more (PubMed: 30910358) »
    • Zhang L  et al. Hsp70 inhibition induces myeloma cell death via the intracellular accumulation of immunoglobulin and the generation of proteotoxic stress. Cancer Lett 339:49-59 (2013). WB ; Human . Read more (PubMed: 23887058) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Question
    Answer

    Thank you for contacting us today.

    I am sorry for the delay in getting back to you. As discussed over the phone, as you intend to use the antibodies ab1939, ab109489 and ab134083 in Western blotting and IHC, we would be able to offer you a testing discount for ab1939 for which we have not tested the antibody in IHC of paraffin embedded sections.

    This offer means that if you purchase ab1939 as normal, test the antibody with your human samples in IHC and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalogue (or the equivalent price of ab1939 off any product). More information on this offer can be found here:

    www.abcam.com/collaborationdiscount

    Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

    If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab1939.



    I am still looking into which positive control tissue to recommend with ab1939. Once I have this information I will get back to you.

    I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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