Key features and details
- Goat polyclonal to Kappa light chain (Biotin)
- Suitable for: Flow Cyt, ELISA, ICC/IF, WB, Dot blot
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Product nameAnti-Kappa light chain antibody (Biotin)
See all Kappa light chain primary antibodies
DescriptionGoat polyclonal to Kappa light chain (Biotin)
SpecificityReacts with human kappa light chains as demonstrated by ELISA and flow cytometry. Cross Absorption: Pooled human myeloma proteins with kappa light chains and mouse immunoglobulins.
Tested applicationsSuitable for: Flow Cyt, ELISA, ICC/IF, WB, Dot blotmore details
Species reactivityReacts with: Human
Human myeloma proteins with kappa light chains
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAffinity chromatography on pooled human immunoglobulins with kappa light chains covalently linked to agarose.
Our Abpromise guarantee covers the use of ab46807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab37376 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
|ELISA||1/5000 - 1/20000.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.|
|Dot blot||Use at an assay dependent concentration.|
RelevanceImmunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
Cellular localizationCytoplasmic and Secreted
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
ab46807 has not yet been referenced specifically in any publications.