Product nameAnti-Kappa light chain antibody [EPR5367-8]
See all Kappa light chain primary antibodies
DescriptionRabbit monoclonal [EPR5367-8] to Kappa light chain
Tested applicationsSuitable for: WB, IP, ELISA, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Full length native protein (purified) corresponding to Human Kappa light chain. Purified Human IgA.
- WB: Human tonsil, plasma and spleen tissue lysates. IHC-P: Human tonsil tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124727 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 25 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
antigen retrieval is recommended.
|ICC/IF||1/500 - 1/1000.|
RelevanceImmunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
Cellular localizationCytoplasmic and Secreted
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
All lanes : Anti-Kappa light chain antibody [EPR5367-8] (ab124727)
Lane 1 : Human tonsil cell lysate
Lane 2 : Human plasma cell lysate
Lane 3 : Human spleen cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 25 kDa
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using ab124727 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab124727 has not yet been referenced specifically in any publications.