Recombinant
RabMAb

Recombinant Anti-Kappa light chain antibody [EPR5367-8] (ab124727)

Overview

  • Product name

    Anti-Kappa light chain antibody [EPR5367-8]
    See all Kappa light chain primary antibodies
  • Description

    Rabbit monoclonal [EPR5367-8] to Kappa light chain
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Kappa light chain. Purified Human IgA.

  • Positive control

    • WB: Human tonsil, plasma and spleen tissue lysates. IHC-P: Human tonsil tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5367-8
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124727 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 25 kDa.
IP 1/10 - 1/100.
ELISA 1/16000.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

antigen retrieval is recommended.

ICC/IF 1/500 - 1/1000.

Target

  • Relevance

    Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
  • Cellular localization

    Cytoplasmic and Secreted
  • Database links

  • Alternative names

    • HCAK 1 antibody
    • HCAK1 antibody
    • Ig kappa chain C region antibody
    • IGKC antibody
    • IGKCD antibody
    • Immunoglobulin InV antibody
    • Immunoglobulin kappa constant antibody
    • Immunoglobulin kappa constant region antibody
    • Immunoglobulin kappa light chain antibody
    • Immunoglobulin kappa light chain constant region antibody
    • Immunoglobulin KM antibody
    • Kappa 1 immunoglobulin light chain antibody
    • kappa light chain antibody
    • Km antibody
    • MGC111575 antibody
    • MGC62011 antibody
    • MGC72072 antibody
    • MGC88770 antibody
    • MGC88771 antibody
    • MGC88809 antibody
    see all

Images

  • All lanes : Anti-Kappa light chain antibody [EPR5367-8] (ab124727)

    Lane 1 : Human tonsil cell lysate
    Lane 2 : Human plasma cell lysate
    Lane 3 : Human spleen cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 25 kDa

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using ab124727 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

References

ab124727 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Merci de nous avoir contactés.

Nous avons un anticorps semblable à l'anticorps A7164. C'est un monoclonal de lapin anti-Human IgG Kappa Light Chain, clone[EPR5367], référence ab124727, https://www.abcam.com/ab124727. Notez que cet anticorps n'est non-conjugué.

Nous avons également d'autres anti-IgG humain, non-conjugués ou conjugués:


https://www.abcam.com/Human-IgG-antibody-ab2410.html
https://www.abcam.com/Human-IgG-antibody-ab2410.html
Rabbit polyclonal

https://www.abcam.com/Human-IgG-antibody-EPR4421-ab109489.htmlhttps://www.abcam.com/Human-IgG-antibody-EPR4421-ab109489.html
https://www.abcam.com/Human-IgG-antibody-EPR4421-ab109489.html
Rabbit monoclonal

https://www.abcam.com/Human-IgG-antibody-2A11-ab7497.html
https://www.abcam.com/Human-IgG-antibody-2A11-ab7497.html
Mouse monoclonal

https://www.abcam.com/Human-IgG-antibody-FITC-ab4220.html
https://www.abcam.com/Human-IgG-antibody-FITC-ab4220.html
Rabbit polyclonal
FITC conjugated

https://www.abcam.com/Human-IgG-antibody-prediluted-ab938.html
https://www.abcam.com/Human-IgG-antibody-prediluted-ab938.html
Rabbit polyclonal

https://www.abcam.com/Human-IgG-antibody-FITC-ab81051.html
https://www.abcam.com/Human-IgG-antibody-FITC-ab81051.html
Goat polyclonal
FITC conjugated

https://www.abcam.com/Human-IgG-antibody-08C05-ab72528.html
https://www.abcam.com/Human-IgG-antibody-08C05-ab72528.html
Mouse monoclonal

https://www.abcam.com/Human-IgG-antibody-CH1-ab7500.html
https://www.abcam.com/Human-IgG-antibody-CH1-ab7500.html
Mouse monoclonal

https://www.abcam.com/Human-IgG-antibody-3D3-ab7498.html
https://www.abcam.com/Human-IgG-antibody-3D3-ab7498.html
Mouse monoclonal

https://www.abcam.com/human-IgG-Fab-fragment-antibody-4A11-ab771.html
https://www.abcam.com/human-IgG-Fab-fragment-antibody-4A11-ab771.html
Mouse monoclonal

https://www.abcam.com/Human-IgG-Lambda-Light-Chain-antibody-EPR5367-ab124719.htmlhttps://www.abcam.com/Human-IgG-Lambda-Light-Chain-antibody-EPR5367-ab124719.html
https://www.abcam.com/Human-IgG-Lambda-Light-Chain-antibody-EPR5367-ab124719.html
Rabbit monoclonal

https://www.abcam.com/Human-IgG-Fc-antibody-ABM122-ab125258.html
https://www.abcam.com/Human-IgG-Fc-antibody-ABM122-ab125258.html
Mouse monoclonal

https://www.abcam.com/Human-Kappa-Chain-antibody-EPR5539-ab134929.htmlhttps://www.abcam.com/Human-Kappa-Chain-antibody-EPR5539-ab134929.html
https://www.abcam.com/Human-Kappa-Chain-antibody-EPR5539-ab134929.html
Rabbit monoclonal

https://www.abcam.com/Human-Kappa-Chain-antibody-SB81a-Biotin-ab79114.html
https://www.abcam.com/Human-Kappa-Chain-antibody-SB81a-Biotin-ab79114.html
Mouse monoclonal
Biotin conjugated

https://www.abcam.com/Human-Kappa-Chain-antibody-SB81a-HRP-ab79115.html
https://www.abcam.com/Human-Kappa-Chain-antibody-SB81a-HRP-ab79115.html
Mouse monoclonal
HRP conjugated

https://www.abcam.com/Human-Kappa-Chain-antibody-SB81a-Alkaline-Phosphatase-ab79117.html
https://www.abcam.com/Human-Kappa-Chain-antibody-SB81a-Alkaline-Phosphatase-ab79117.html
Mouse monoclonal
Alkaline Phosphatase conjugated

https://www.abcam.com/Human-Kappa-Chain-antibody-SB81a-FITC-ab79118.html
https://www.abcam.com/Human-Kappa-Chain-antibody-SB81a-FITC-ab79118.html
Mouse monoclonal
FITC conjugated

https://www.abcam.com/Human-Kappa-Chain-antibody-SB81a-Allophycocyanin-ab79112.html
https://www.abcam.com/Human-Kappa-Chain-antibody-SB81a-Allophycocyanin-ab79112.html
Mouse monoclonal
Allophycocyanin conjugated

https://www.abcam.com/Human-Kappa-Chain-antibody-SB81a-Phycoerythrin-ab79113.html
https://www.abcam.com/Human-Kappa-Chain-antibody-SB81a-Phycoerythrin-ab79113.html
Mouse monoclonal
Phycoerythrin conjugated






Les prix et les délais de livraison apparaissent sur chacune des fiches techniques.

J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up