RecombinantRabMAb

Recombinant Anti-Kappa light chain antibody [EPR5539-105-4] (ab134930)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5539-105-4] to Kappa light chain
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Kappa light chain antibody [EPR5539-105-4]
    See all Kappa light chain primary antibodies

  • Description

    Rabbit monoclonal [EPR5539-105-4] to Kappa light chain

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Other Immunogen Type corresponding to Human Kappa light chain.

  • Positive control

    • Human tonsil, Human spleen and Human plasma lysates. Human tonsil and B cell lymphoma tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR5539-105-4

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 49 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for IP.

    • Target

    • Relevance

      Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.

    • Cellular localization

      Cytoplasmic and Secreted

    • Database links

      • Alternative names

        • HCAK 1 antibody
        • HCAK1 antibody
        • Ig kappa chain C region antibody
        • IGKC antibody
        • IGKCD antibody
        • Immunoglobulin InV antibody
        • Immunoglobulin kappa constant antibody
        • Immunoglobulin kappa constant region antibody
        • Immunoglobulin kappa light chain antibody
        • Immunoglobulin kappa light chain constant region antibody
        • Immunoglobulin KM antibody
        • Kappa 1 immunoglobulin light chain antibody
        • kappa light chain antibody
        • Km antibody
        • MGC111575 antibody
        • MGC62011 antibody
        • MGC72072 antibody
        • MGC88770 antibody
        • MGC88771 antibody
        • MGC88809 antibody
        see all

      Images

      • Western blot - Anti-Kappa light chain antibody [EPR5539-105-4] (ab134930)
        Western blot - Anti-Kappa light chain antibody [EPR5539-105-4] (ab134930)
        All lanes : Anti-Kappa light chain antibody [EPR5539-105-4] (ab134930) at 1/10000 dilution

        Lane 1 : Human tonsil lysate
        Lane 2 : Human spleen lysate
        Lane 3 : Human plasma lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 49 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [EPR5539-105-4] (ab134930)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [EPR5539-105-4] (ab134930)

        Immunohistochemical analysis of paraffin-embedded Human tonsil tissue staining IgM with ab134930 at 1/500 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [EPR5539-105-4] (ab134930)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [EPR5539-105-4] (ab134930)

        Immunohistochemical analysis of paraffin-embedded B cell lymphoma tissue staining IgM with ab134930 at 1/500 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [EPR5539-105-4] (ab134930)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [EPR5539-105-4] (ab134930)

        Immunohistochemical analysis using ab134930 showing positive staining in Normal colon, lymphocytes tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [EPR5539-105-4] (ab134930)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa light chain antibody [EPR5539-105-4] (ab134930)

        Immunohistochemical analysis using ab134930 showing positive staining in Normal stomach, lymphocytes tissue.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Anti-Kappa light chain antibody [EPR5539-105-4] (ab134930)
        Anti-Kappa light chain antibody [EPR5539-105-4] (ab134930)

      References (1)

      Publishing research using ab134930? Please let us know so that we can cite the reference in this datasheet.

      ab134930 has been referenced in 1 publication.

      • Lv L  et al. Arbutin protects HK-2 cells against high glucose-induced apoptosis and autophagy by up-regulating microRNA-27a. Artif Cells Nanomed Biotechnol 47:2940-2947 (2019). PubMed: 31319730

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