Key features and details
- Rabbit polyclonal to Kappa light chain (FITC)
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG
Product nameAnti-Kappa light chain antibody (FITC)
See all Kappa light chain primary antibodies
DescriptionRabbit polyclonal to Kappa light chain (FITC)
ConjugationFITC. Ex: 493nm, Em: 528nm
Tested applicationsSuitable for: IHC-P, IHC-Frmore details
Species reactivityReacts with: Human
Pool of human kappa light chain isolated from the urine of patients with Bence Jones Proteinuria.
Fluorescein isothiocyanate (FITC) isomer 1.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 1% BSA
Concentration information loading...
Purification notesTraces of contaminating antibodies have been removed by solid phase absorption. Only the kappa precipitation line appeared in cross immunoelectrophoresis.
Our Abpromise guarantee covers the use of ab4208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/40. Incubation for 30-60 minutes at room temperature is suggested.|
|IHC-Fr||Use at an assay dependent dilution.|
RelevanceImmunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
Cellular localizationCytoplasmic and Secreted
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
ab4208 has not yet been referenced specifically in any publications.