Key features and details
- Goat polyclonal to Kappa light chain (HRP)
- Suitable for: IHC-Fr, ELISA, WB
- Reacts with: Mouse
- Conjugation: HRP
- Isotype: IgG
Product nameAnti-Kappa light chain antibody (HRP)
See all Kappa light chain primary antibodies
DescriptionGoat polyclonal to Kappa light chain (HRP)
SpecificityThis antibody recognises mouse kappa light chains; both bound and free.
Tested applicationsSuitable for: IHC-Fr, ELISA, WBmore details
Species reactivityReacts with: Mouse
Mouse paraproteins with kappa light chains
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferConstituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Purification notesThis antibody was made from pooled antisera from goats immunised with mouse paraproteins bearing kappa light chains. Antiserum was purified by affinity chromatography on pooled mouse Igs with kappa light chains. The antiserum was cross adsorbed on pooled mouse Igs with lambda light chains.
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab46527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent dilution.|
|ELISA||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution. Predicted molecular weight: 12 kDa.|
RelevanceImmunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
Cellular localizationCytoplasmic and Secreted
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
ab46527 has not yet been referenced specifically in any publications.