HRP Anti-Kappa light chain antibody (ab46527)
Key features and details
- HRP Goat polyclonal to Kappa light chain
- Suitable for: WB, IHC-Fr, ELISA
- Reacts with: Mouse
- Conjugation: HRP
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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- Ethical standards compliant – production is animal-free
Overview
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Product name
HRP Anti-Kappa light chain antibody
See all Kappa light chain primary antibodies -
Description
HRP Goat polyclonal to Kappa light chain -
Host species
Goat -
Conjugation
HRP -
Specificity
This antibody recognises mouse kappa light chains; both bound and free. -
Tested applications
Suitable for: WB, IHC-Fr, ELISAmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Full length protein corresponding to Mouse Kappa light chain. Mouse paraproteins with kappa light chains
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
This antibody was made from pooled antisera from goats immunised with mouse paraproteins bearing kappa light chains. Antiserum was purified by affinity chromatography on pooled mouse Igs with kappa light chains. The antiserum was cross adsorbed on pooled mouse Igs with lambda light chains. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab46527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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Notes |
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WB
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
Target
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Relevance
Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83. -
Cellular localization
Cytoplasmic and Secreted -
Database links
- Entrez Gene: 16071 Mouse
- SwissProt: P01837 Mouse
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Alternative names
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab46527 has not yet been referenced specifically in any publications.