Key features and details
- Mouse monoclonal [KLC264] to Kappa light chain
- Suitable for: Flow Cyt, IP, IHC-Fr, WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Kappa light chain antibody [KLC264]
See all Kappa light chain primary antibodies
DescriptionMouse monoclonal [KLC264] to Kappa light chain
Tested applicationsSuitable for: Flow Cyt, IP, IHC-Fr, WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human Kappa light chain. Recombinant full length human Kappa light chain corresponding to: Ig kappa chain V-I region HK101 (amino acids 1-117; P01601) and Ig kappa chain C (amino acids 1-106; P01834).
- 293T, Raji or hPBL cells. Tonsil and Spleen tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS
It contains BSA and sodium azide.
Concentration information loading...
PurityProtein A/G purified
Purification notes200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G
Light chain typekappa
Our Abpromise guarantee covers the use of ab190760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 0.5 - 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at 2-4 µg/mg of lysate.|
|IHC-Fr||Use a concentration of 0.5 - 1 µg/ml.|
|WB||1/250 - 1/500. Predicted molecular weight: 23 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceImmunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
Cellular localizationCytoplasmic and Secreted
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
ab190760 has not yet been referenced specifically in any publications.