Anti-Kappa light chain antibody [KLC264] - BSA and Azide free (ab233888)

Overview

  • Product name

    Anti-Kappa light chain antibody [KLC264] - BSA and Azide free
    See all Kappa light chain primary antibodies
  • Description

    Mouse monoclonal [KLC264] to Kappa light chain - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human Kappa light chain. Recombinant full length human Kappa light chain corresponding to: Ig kappa chain V-I region HK101 (amino acids 1-117; P01601) and Ig kappa chain C (amino acids 1-106; P01834).

  • Positive control

    • WB: Human spleen lysate. IHC-P: Human tonsil tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from bioreactor concentrate.
  • Clonality

    Monoclonal
  • Clone number

    KLC264
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab233888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 25 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
  • Cellular localization

    Cytoplasmic and Secreted
  • Database links

  • Alternative names

    • HCAK 1 antibody
    • HCAK1 antibody
    • Ig kappa chain C region antibody
    • IGKC antibody
    • IGKCD antibody
    • Immunoglobulin InV antibody
    • Immunoglobulin kappa constant antibody
    • Immunoglobulin kappa constant region antibody
    • Immunoglobulin kappa light chain antibody
    • Immunoglobulin kappa light chain constant region antibody
    • Immunoglobulin KM antibody
    • Kappa 1 immunoglobulin light chain antibody
    • kappa light chain antibody
    • Km antibody
    • MGC111575 antibody
    • MGC62011 antibody
    • MGC72072 antibody
    • MGC88770 antibody
    • MGC88771 antibody
    • MGC88809 antibody
    see all

Images

  • Anti-Kappa light chain antibody [KLC264] - BSA and Azide free (ab233888) at 1 µg/ml + Human spleen lysate

    Predicted band size: 25 kDa

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for Kappa light chain using ab233888 at 1 μg/ml in immunohistochemical analysis. 

References

ab233888 has not yet been referenced specifically in any publications.

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