Anti-Kappa light chain antibody [MEM-09] (ab1050)
Key features and details
- Mouse monoclonal [MEM-09] to Kappa light chain
- Suitable for: IP, WB, IHC-P, Flow Cyt, ICC
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Kappa light chain antibody [MEM-09]
See all Kappa light chain primary antibodies -
Description
Mouse monoclonal [MEM-09] to Kappa light chain -
Host species
Mouse -
Specificity
MAb MEM-09 reacts with both secreted and B cell-surface human immunoglobulin, specifically reacts with k-light chains. Material immunoprecipitated from human serum using MAb MEM-09 consist of IgG and traces of IgM. -
Tested applications
Suitable for: IP, WB, IHC-P, Flow Cyt, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue/ cell preparation (Human). (Crude thymus membrane fraction).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from TCS. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
MEM-09 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab1050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| Flow Cyt | Use a concentration of 5 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| ICC | Use at an assay dependent concentration. |
Target
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Relevance
Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83. -
Cellular localization
Cytoplasmic and Secreted -
Database links
- Entrez Gene: 28901 Human
- Entrez Gene: 3514 Human
- Omim: 147200 Human
- SwissProt: P01601 Human
- SwissProt: P01834 Human
- Unigene: 449609 Human
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Alternative names
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
see all
Images
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![Western blot - Anti-Kappa light chain antibody [MEM-09] (ab1050)](https://www.abcam.com/ps/products/1/ab1050/Images/ab1050-3950-anti-kappa-light-chain-antibody-mem-09-western-blot.jpg)
Western blot - Anti-Kappa light chain antibody [MEM-09] (ab1050)This image is courtesy of an anonymous AbreviewAnti-Kappa light chain antibody [MEM-09] (ab1050) at 1/2000 dilution + Human B lymphocytes, whole cell lysate at 15 µg
Secondary
Alkaline phosphatase conjugated goat anti-mouse antibody at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 11 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsThe blot was blocked with 2% milk for 2 hours at 22°C and incubated with the primary antibody for 1 hour and 20 minutes at 22°C.
Protocols
References
ab1050 has been referenced in 4 publications.
- Cao J et al. Versatile and on-demand biologics co-production in yeast. Nat Commun 9:77 (2018). PubMed: 29311542
- Shah KA et al. Automated pipeline for rapid production and screening of HIV-specific monoclonal antibodies using pichia pastoris. Biotechnol Bioeng 112:2624-9 (2015). PubMed: 26032261
- O'Callaghan PM et al. Cell line-specific control of recombinant monoclonal antibody production by CHO cells. Biotechnol Bioeng 106:938-51 (2010). WB ; Chinese hamster . PubMed: 20589672
- Horejsi V et al. Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. Folia Biol (Praha) 32:12-25 (1986). PubMed: 2422063
