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    kappa-light-chain-antibody-mem-09-ab1050.pdf

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Immunology Immunoglobulins Light Chain Kappa
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Anti-Kappa light chain antibody [MEM-09] (ab1050)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (4)

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Key features and details

  • Mouse monoclonal [MEM-09] to Kappa light chain
  • Suitable for: IHC-P, ICC, Flow Cyt (Intra), IP, WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Kappa light chain antibody [MEM-09]
    See all Kappa light chain primary antibodies
  • Description

    Mouse monoclonal [MEM-09] to Kappa light chain
  • Host species

    Mouse
  • Specificity

    MAb MEM-09 reacts with both secreted and B cell-surface human immunoglobulin, specifically reacts with k-light chains. Material immunoprecipitated from human serum using MAb MEM-09 consist of IgG and traces of IgM.
  • Tested applications

    Suitable for: IHC-P, ICC, Flow Cyt (Intra), IP, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human Kappa light chain. Crude thymus membrane fraction

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from TCS. Purity >95% by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    MEM-09
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Immunoglobulins
    • Light Chain
    • Kappa

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab1050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP
Use at an assay dependent concentration.
WB (1)
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Notes
IHC-P
Use at an assay dependent concentration.
ICC
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Relevance

    Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
  • Cellular localization

    Cytoplasmic and Secreted
  • Database links

    • Entrez Gene: 28901 Human
    • Entrez Gene: 3514 Human
    • Omim: 147200 Human
    • SwissProt: P01601 Human
    • SwissProt: P01834 Human
    • Alternative names

      • HCAK 1 antibody
      • HCAK1 antibody
      • Ig kappa chain C region antibody
      • IGKC antibody
      • IGKCD antibody
      • Immunoglobulin InV antibody
      • Immunoglobulin kappa constant antibody
      • Immunoglobulin kappa constant region antibody
      • Immunoglobulin kappa light chain antibody
      • Immunoglobulin kappa light chain constant region antibody
      • Immunoglobulin KM antibody
      • Kappa 1 immunoglobulin light chain antibody
      • kappa light chain antibody
      • Km antibody
      • MGC111575 antibody
      • MGC62011 antibody
      • MGC72072 antibody
      • MGC88770 antibody
      • MGC88771 antibody
      • MGC88809 antibody
      see all

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (4)

    Publishing research using ab1050? Please let us know so that we can cite the reference in this datasheet.

    ab1050 has been referenced in 4 publications.

    • Cao J  et al. Versatile and on-demand biologics co-production in yeast. Nat Commun 9:77 (2018). PubMed: 29311542
    • Shah KA  et al. Automated pipeline for rapid production and screening of HIV-specific monoclonal antibodies using pichia pastoris. Biotechnol Bioeng 112:2624-9 (2015). PubMed: 26032261
    • O'Callaghan PM  et al. Cell line-specific control of recombinant monoclonal antibody production by CHO cells. Biotechnol Bioeng 106:938-51 (2010). WB ; Chinese hamster . PubMed: 20589672
    • Horejsi­ V  et al. Monoclonal antibodies against human leucocyte antigens. I. Antibodies against beta-2-microglobulin, immunoglobulin kappa light chains, HLA-DR-like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan-leucocyte antigen. Folia Biol (Praha) 32:12-25 (1986). PubMed: 2422063

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-Kappa light chain antibody [MEM-09]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (B lymphocytes)
    Loading amount
    15 µg
    Specification
    B lymphocytes
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 06 2008

    Question

    which dilution do you use for mem09 used in WB??? always 1ug/ml

    Read More

    Abcam community

    Verified customer

    Asked on Aug 06 2002

    Answer

    Since the immunoglobulin concentration of this product is 1mg/ml, I would suggest a starting concentration of 1ug/ml. Adjust this accordingly from the results obtained.

    Read More

    Abcam Scientific Support

    Answered on Aug 07 2002

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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