Product nameAnti-Kappa light chain antibody [RM103]
See all Kappa light chain primary antibodies
DescriptionRabbit monoclonal [RM103] to Kappa light chain
Tested applicationsSuitable for: IP, WB, ELISA, IHC-P, Flow Cyt, ICCmore details
Species reactivityReacts with: Mouse
Full length protein corresponding to Mouse Kappa light chain. (Mouse IgG).
- Mouse IgG1 kappa.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% PBS, 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesab190484 was purified from an animal origin–free and protein-free culture supernatant.
Our Abpromise guarantee covers the use of ab190484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Use under non reducing condition. Predicted molecular weight: 25 kDa.|
|ELISA||Use a concentration of 0.005 - 0.2 µg/ml.|
|IHC-P||Use a concentration of 0.5 - 2 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC||Use a concentration of 0.5 - 2 µg/ml.|
RelevanceImmunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
Cellular localizationCytoplasmic and Secreted
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
All lanes : Anti-Kappa light chain antibody [RM103] (ab190484) at 0.2 µg/ml
Lane 1 : Non-reduced Mouse IgG1 kappa
Lane 2 : Reduced Mouse IgG1 kappa
Lane 3 : Non-reduced Mouse IgG1 lambda
Lane 4 : Reduced Mouse IgG1 lambda
Lysates/proteins at 0.02 µg per lane.
Predicted band size: 25 kDa
ab190484 reacted to Non-reduced IgG1 kappa (~150 kDa), and slightly reacted to reduced kappa light chain (~25 kDa).
ELISA of mouse immunoglobulins shows ab190484 reacted to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab190484 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.
A titer ELISA of mouse IgG1 kappa. The plate was coated with different amounts of mouse IgG1 kappa. A serial dilution of ab190484 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.
ab190484 has not yet been referenced specifically in any publications.