Recombinant

Recombinant Anti-Kappa light chain antibody [RM103] (ab190484)

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Western blot - Anti-Kappa light chain antibody [RM103] (ab190484)
  • ELISA - Anti-Kappa light chain antibody [RM103] (ab190484)
  • ELISA - Anti-Kappa light chain antibody [RM103] (ab190484)
  • Anti-Kappa light chain antibody [RM103] (ab190484)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [RM103] to Kappa light chain
  • Suitable for: WB, ELISA
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Kappa light chain antibody [RM103]
    See all Kappa light chain primary antibodies

  • Description

    Rabbit monoclonal [RM103] to Kappa light chain

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Full length protein corresponding to Mouse Kappa light chain. (Mouse IgG).

  • Positive control

    • Mouse IgG1 kappa.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 48.91% PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    ab190484 was purified from an animal origin–free and protein-free culture supernatant.

  • Clonality

    Monoclonal

  • Clone number

    RM103

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab190484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Use under non reducing condition. Predicted molecular weight: 25 kDa.
ELISA Use a concentration of 0.005 - 0.2 µg/ml.

Target

  • Relevance

    Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.

  • Cellular localization

    Cytoplasmic and Secreted

  • Database links

    • Alternative names

      • HCAK 1 antibody
      • HCAK1 antibody
      • Ig kappa chain C region antibody
      • IGKC antibody
      • IGKCD antibody
      • Immunoglobulin InV antibody
      • Immunoglobulin kappa constant antibody
      • Immunoglobulin kappa constant region antibody
      • Immunoglobulin kappa light chain antibody
      • Immunoglobulin kappa light chain constant region antibody
      • Immunoglobulin KM antibody
      • Kappa 1 immunoglobulin light chain antibody
      • kappa light chain antibody
      • Km antibody
      • MGC111575 antibody
      • MGC62011 antibody
      • MGC72072 antibody
      • MGC88770 antibody
      • MGC88771 antibody
      • MGC88809 antibody
      see all

    Images

    • Western blot - Anti-Kappa light chain antibody [RM103] (ab190484)
      Western blot - Anti-Kappa light chain antibody [RM103] (ab190484)
      All lanes : Anti-Kappa light chain antibody [RM103] (ab190484) at 0.2 µg/ml

      Lane 1 : Non-reduced Mouse IgG1 kappa
      Lane 2 : Reduced Mouse IgG1 kappa
      Lane 3 : Non-reduced Mouse IgG1 lambda
      Lane 4 : Reduced Mouse IgG1 lambda

      Lysates/proteins at 0.02 µg per lane.

      Predicted band size: 25 kDa



      ab190484 reacted to Non-reduced IgG1 kappa (~150 kDa), and slightly reacted to reduced kappa light chain (~25 kDa).

    • ELISA - Anti-Kappa light chain antibody [RM103] (ab190484)
      ELISA - Anti-Kappa light chain antibody [RM103] (ab190484)

      ELISA of mouse immunoglobulins shows ab190484 reacted to the kappa light chain of mouse immunoglobulins. No cross reactivity with the lamda light chain, human IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab190484 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.

    • ELISA - Anti-Kappa light chain antibody [RM103] (ab190484)
      ELISA - Anti-Kappa light chain antibody [RM103] (ab190484)

      A titer ELISA of mouse IgG1 kappa. The plate was coated with different amounts of mouse IgG1 kappa. A serial dilution of ab190484 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.

    • Anti-Kappa light chain antibody [RM103] (ab190484)
      Anti-Kappa light chain antibody [RM103] (ab190484)

    References (1)

    Publishing research using ab190484? Please let us know so that we can cite the reference in this datasheet.

    ab190484 has been referenced in 1 publication.

    • Deng Q  et al. Tissue-specific microRNA expression alters cancer susceptibility conferred by a TP53 noncoding variant. Nat Commun 10:5061 (2019). PubMed: 31699989

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