Recombinant
RabMAb

Recombinant Anti-Kappa light chain antibody [SP148] (ab227654)

Overview

  • Product name

    Anti-Kappa light chain antibody [SP148]
    See all Kappa light chain primary antibodies
  • Description

    Rabbit monoclonal [SP148] to Kappa light chain
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Other Immunogen Type corresponding to Human Kappa light chain. Free kappa light chain from pooled human IgG lambda myeloma sera.

  • Positive control

    • IHC-P: Human tonsil, colon, stomach, spleen, follicular lymphoma, and B-cell lymphoma tissue; WB: Human spleen lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP148
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.

For antigen retrieval: Boil tissue section in 1mM EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes before commencing with IHC staining protocol.

WB 1/100. Predicted molecular weight: 25 kDa.

Target

  • Relevance

    Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
  • Cellular localization

    Cytoplasmic and Secreted
  • Database links

  • Alternative names

    • HCAK 1 antibody
    • HCAK1 antibody
    • Ig kappa chain C region antibody
    • IGKC antibody
    • IGKCD antibody
    • Immunoglobulin InV antibody
    • Immunoglobulin kappa constant antibody
    • Immunoglobulin kappa constant region antibody
    • Immunoglobulin kappa light chain antibody
    • Immunoglobulin kappa light chain constant region antibody
    • Immunoglobulin KM antibody
    • Kappa 1 immunoglobulin light chain antibody
    • kappa light chain antibody
    • Km antibody
    • MGC111575 antibody
    • MGC62011 antibody
    • MGC72072 antibody
    • MGC88770 antibody
    • MGC88771 antibody
    • MGC88809 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Kappa light chain with Purified ab227654 at 1/100 dilution (0.90 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Anti-Kappa light chain antibody [SP148] (ab227654) at 1/100 dilution + Human spleen lysate

    Predicted band size: 25 kDa

  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for Kappa light chain using ab227654 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon tissue stained for Kappa light chain using ab227654 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human stomach tissue stained for Kappa light chain using ab227654 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human follicular lymphoma tissue stained for Kappa light chain using ab227654 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human B-cell lymphoma tissue stained for Kappa light chain using ab227654 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human spleen tissue stained for Kappa light chain using ab227654 at 1/100 dilution in immunohistochemical analysis.

References

ab227654 has not yet been referenced specifically in any publications.

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