APC Anti-Kappa light chain antibody [TB28-2] (ab119122)
Key features and details
- APC Mouse monoclonal [TB28-2] to Kappa light chain
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
- Isotype: IgG1
Overview
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Product name
APC Anti-Kappa light chain antibody [TB28-2]
See all Kappa light chain primary antibodies -
Description
APC Mouse monoclonal [TB28-2] to Kappa light chain -
Host species
Mouse -
Conjugation
APC. Ex: 645nm, Em: 660nm -
Specificity
ab119122 recognizes Human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Human IgG-kappa myeloma protein.
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Positive control
- Human blood cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS, 0.2% BSA
BSA (high-grade protease free) -
Concentration information loading...
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Purity
Size exclusion -
Purification notes
ab119122 is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. -
Clonality
Monoclonal -
Clone number
TB28-2 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 10µg for 106 cells.
ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use 10µg for 106 cells. ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83. -
Cellular localization
Cytoplasmic and Secreted -
Database links
- Entrez Gene: 28901 Human
- Entrez Gene: 3514 Human
- Omim: 147200 Human
- SwissProt: P01601 Human
- SwissProt: P01834 Human
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Alternative names
- HCAK 1 antibody
- HCAK1 antibody
- Ig kappa chain C region antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab119122 has not yet been referenced specifically in any publications.