Anti-Kappa light chain antibody [TB28-2] (Allophycocyanin) (ab119122)

Overview

  • Product name

    Anti-Kappa light chain antibody [TB28-2] (Allophycocyanin)
    See all Kappa light chain primary antibodies
  • Description

    Mouse monoclonal [TB28-2] to Kappa light chain (Allophycocyanin)
  • Host species

    Mouse
  • Conjugation

    Allophycocyanin. Ex: 645nm, Em: 660nm
  • Specificity

    ab119122 recognizes Human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains.
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Human IgG-kappa myeloma protein.

  • Positive control

    • Human blood cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 99% PBS, 0.2% BSA

    BSA (high-grade protease free)
  • Concentration information loading...
  • Purity

    Size exclusion
  • Purification notes

    ab119122 is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
  • Clonality

    Monoclonal
  • Clone number

    TB28-2
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab119122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

ab37391 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance

    Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain. The EU sequence has the INV allotypic marker, Ala 45 and Val 83. The ROY sequence has the INV allotypic marker, Ala 45 and Leu 83.
  • Cellular localization

    Cytoplasmic and Secreted
  • Database links

  • Alternative names

    • HCAK 1 antibody
    • HCAK1 antibody
    • Ig kappa chain C region antibody
    • IGKC antibody
    • IGKCD antibody
    • Immunoglobulin InV antibody
    • Immunoglobulin kappa constant antibody
    • Immunoglobulin kappa constant region antibody
    • Immunoglobulin kappa light chain antibody
    • Immunoglobulin kappa light chain constant region antibody
    • Immunoglobulin KM antibody
    • Kappa 1 immunoglobulin light chain antibody
    • kappa light chain antibody
    • Km antibody
    • MGC111575 antibody
    • MGC62011 antibody
    • MGC72072 antibody
    • MGC88770 antibody
    • MGC88771 antibody
    • MGC88809 antibody
    see all

References

ab119122 has not yet been referenced specifically in any publications.

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