Recombinant Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18881] to Kappa Opioid Receptor
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-Kappa Opioid Receptor antibody [EPR18881]
See all Kappa Opioid Receptor primary antibodies -
Description
Rabbit monoclonal [EPR18881] to Kappa Opioid Receptor -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse and rat brain lysates. IHC-P: Mouse and rat hippocampus tissues. IP: Mouse brain whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18881 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183825 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/50.
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IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 43 kDa).
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Notes |
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IP
1/50. |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 43 kDa). |
Target
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Function
Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for dynorphins. May play a role in arousal and regulation of autonomic and neuroendocrine functions. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 18387 Mouse
- Entrez Gene: 29335 Rat
- SwissProt: P33534 Mouse
- SwissProt: P34975 Rat
- Unigene: 7977 Mouse
- Unigene: 89571 Rat
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Alternative names
- K-OR-1 antibody
- Kappa opioid receptor antibody
- Kappa type opioid receptor antibody
see all
Images
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All lanes : Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lane 6 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 43 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID:20574683).
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All lanes : Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat heart lysate
Lane 3 : Rat kidney lysate
Lane 4 : Rat spleen lysate
Lane 5 : C6 (Rat glial tumor cells) whole cell lysate
Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 43 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID:20574683).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Kappa Opioid Receptor with ab183825 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Mainly membrane staining on neuron cell of mouse hippocampus is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Kappa Opioid Receptor with ab183825 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Negative staining on mouse spleen.
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling Kappa Opioid Receptor with ab183825 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Mainly membrane staining on neuron cells of rat hippocampus is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Kappa Opioid Receptor with ab183825 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Negative staining on rat spleen.
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Kappa Opioid Receptor was immunoprecipitated from 1mg of Mouse brain whole cell lysate with ab183825 at 1/100 dilution.
Western blot was performed from the immunoprecipitate using ab183825 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse brain whole cell lysate 10µg (Input).
Lane 2: ab183825 IP in Mouse brain whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab183825 in Mouse brain whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (4)
ab183825 has been referenced in 4 publications.
- Jin ZR et al. Roles of CatSper channels in the pathogenesis of asthenozoospermia and the therapeutic effects of acupuncture-like treatment on asthenozoospermia. Theranostics 11:2822-2844 (2021). PubMed: 33456575
- Blackwood CA et al. Molecular Adaptations in the Rat Dorsal Striatum and Hippocampus Following Abstinence-Induced Incubation of Drug Seeking After Escalated Oxycodone Self-Administration. Mol Neurobiol 56:3603-3615 (2019). PubMed: 30155791
- Zhang K et al. Methionine enkephalin (MENK) regulates the immune pathogenesis of type 2 diabetes mellitus via the IL-33/ST2 pathway. Int Immunopharmacol 73:23-40 (2019). PubMed: 31078923
- Blackwood CA et al. Escalated Oxycodone Self-Administration and Punishment: Differential Expression of Opioid Receptors and Immediate Early Genes in the Rat Dorsal Striatum and Prefrontal Cortex. Front Neurosci 13:1392 (2019). PubMed: 31998063