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    kappa-opioid-receptor-antibody-epr18881-ab183825.pdf

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RecombinantRabMAb

Recombinant Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question References (4)

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Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
  • Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
  • Immunoprecipitation - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
  • Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18881] to Kappa Opioid Receptor
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Kappa Opioid Receptor antibody [EPR18881]
    See all Kappa Opioid Receptor primary antibodies
  • Description

    Rabbit monoclonal [EPR18881] to Kappa Opioid Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat brain lysates. IHC-P: Mouse and rat hippocampus tissues. IP: Mouse brain whole cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18881
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Opioid Receptors
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Kappa Opioid Receptor antibody [EPR18881] - BSA and Azide free (ab240278)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Kappa Opioid Receptor protein (ab114296)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab183825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/50.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 43 kDa).
Notes
IP
1/50.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 43 kDa).

Target

  • Function

    Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for dynorphins. May play a role in arousal and regulation of autonomic and neuroendocrine functions.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P41145 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 18387 Mouse
    • Entrez Gene: 29335 Rat
    • SwissProt: P33534 Mouse
    • SwissProt: P34975 Rat
    • Unigene: 7977 Mouse
    • Unigene: 89571 Rat
    • Alternative names

      • K-OR-1 antibody
      • Kappa opioid receptor antibody
      • Kappa type opioid receptor antibody
      • Kappa-type opioid receptor antibody
      • KOR 1 antibody
      • KOR antibody
      • KOR-1 antibody
      • Opiate receptor kappa 1 antibody
      • Opiate receptor, kappa 1 antibody
      • Opioid receptor, kappa 1 antibody
      • OPRK 1 antibody
      • OPRK antibody
      • OPRK_HUMAN antibody
      • OPRK1 antibody
      • Oprk2 antibody
      • R21 antibody
      see all

    Images

    • Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
      Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
      All lanes : Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Mouse heart lysate
      Lane 3 : Mouse kidney lysate
      Lane 4 : Mouse spleen lysate
      Lane 5 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
      Lane 6 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 43 kDa
      Observed band size: 58 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID:20574683).

    • Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
      Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
      All lanes : Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825) at 1/1000 dilution

      Lane 1 : Rat brain lysate
      Lane 2 : Rat heart lysate
      Lane 3 : Rat kidney lysate
      Lane 4 : Rat spleen lysate
      Lane 5 : C6 (Rat glial tumor cells) whole cell lysate
      Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 43 kDa
      Observed band size: 58 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID:20574683).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)

      Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Kappa Opioid Receptor with ab183825 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Mainly membrane staining on neuron cell of mouse hippocampus is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)

      Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Kappa Opioid Receptor with ab183825 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Negative staining on mouse spleen.

      Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)

      Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling Kappa Opioid Receptor with ab183825 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Mainly membrane staining on neuron cells of rat hippocampus is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)

      Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Kappa Opioid Receptor with ab183825 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Negative staining on rat spleen.

      Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
      Immunoprecipitation - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)

      Kappa Opioid Receptor was immunoprecipitated from 1mg of Mouse brain whole cell lysate with ab183825 at 1/100 dilution.

      Western blot was performed from the immunoprecipitate using ab183825 at 1/1000 dilution.

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: Mouse brain whole cell lysate 10µg (Input).

      Lane 2: ab183825 IP in Mouse brain whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab183825 in Mouse brain whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 5 seconds.

    • Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
      Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (4)

    Publishing research using ab183825? Please let us know so that we can cite the reference in this datasheet.

    ab183825 has been referenced in 4 publications.

    • Jin ZR  et al. Roles of CatSper channels in the pathogenesis of asthenozoospermia and the therapeutic effects of acupuncture-like treatment on asthenozoospermia. Theranostics 11:2822-2844 (2021). PubMed: 33456575
    • Blackwood CA  et al. Molecular Adaptations in the Rat Dorsal Striatum and Hippocampus Following Abstinence-Induced Incubation of Drug Seeking After Escalated Oxycodone Self-Administration. Mol Neurobiol 56:3603-3615 (2019). PubMed: 30155791
    • Zhang K  et al. Methionine enkephalin (MENK) regulates the immune pathogenesis of type 2 diabetes mellitus via the IL-33/ST2 pathway. Int Immunopharmacol 73:23-40 (2019). PubMed: 31078923
    • Blackwood CA  et al. Escalated Oxycodone Self-Administration and Punishment: Differential Expression of Opioid Receptors and Immediate Early Genes in the Rat Dorsal Striatum and Prefrontal Cortex. Front Neurosci 13:1392 (2019). PubMed: 31998063

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