Recombinant
RabMAb

Recombinant Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)

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Overview

  • Product name

    Anti-Kappa Opioid Receptor antibody [EPR18881]
    See all Kappa Opioid Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR18881] to Kappa Opioid Receptor

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Mouse Kappa Opioid Receptor aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: P33534

  • Positive control

    • WB: Mouse and rat brain lysates. IHC-P: Mouse and rat hippocampus tissues. IP: Mouse brain whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18881

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/50.
IHC-P 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 43 kDa).

Target

  • Function

    Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for dynorphins. May play a role in arousal and regulation of autonomic and neuroendocrine functions.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • K-OR-1 antibody
      • Kappa opioid receptor antibody
      • Kappa type opioid receptor antibody
      • Kappa-type opioid receptor antibody
      • KOR 1 antibody
      • KOR antibody
      • KOR-1 antibody
      • Opiate receptor kappa 1 antibody
      • Opiate receptor, kappa 1 antibody
      • Opioid receptor, kappa 1 antibody
      • OPRK 1 antibody
      • OPRK antibody
      • OPRK_HUMAN antibody
      • OPRK1 antibody
      • Oprk2 antibody
      • R21 antibody
      see all

    Images

    • Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
      Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
      All lanes : Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825) at 1/1000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Mouse heart lysate
      Lane 3 : Mouse kidney lysate
      Lane 4 : Mouse spleen lysate
      Lane 5 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
      Lane 6 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 43 kDa
      Observed band size: 58 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID:20574683).

    • Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
      Western blot - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
      All lanes : Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825) at 1/1000 dilution

      Lane 1 : Rat brain lysate
      Lane 2 : Rat heart lysate
      Lane 3 : Rat kidney lysate
      Lane 4 : Rat spleen lysate
      Lane 5 : C6 (Rat glial tumor cells) whole cell lysate
      Lane 6 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 43 kDa
      Observed band size: 58 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID:20574683).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)

      Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Kappa Opioid Receptor with ab183825 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Mainly membrane staining on neuron cell of mouse hippocampus is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)

      Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Kappa Opioid Receptor with ab183825 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Negative staining on mouse spleen.

      Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)

      Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling Kappa Opioid Receptor with ab183825 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Mainly membrane staining on neuron cells of rat hippocampus is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)

      Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Kappa Opioid Receptor with ab183825 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Negative staining on rat spleen.

      Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)
      Immunoprecipitation - Anti-Kappa Opioid Receptor antibody [EPR18881] (ab183825)

      Kappa Opioid Receptor was immunoprecipitated from 1mg of Mouse brain whole cell lysate with ab183825 at 1/100 dilution.

      Western blot was performed from the immunoprecipitate using ab183825 at 1/1000 dilution.

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: Mouse brain whole cell lysate 10µg (Input).

      Lane 2: ab183825 IP in Mouse brain whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab183825 in Mouse brain whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 5 seconds.

    References

    Publishing research using ab183825? Please let us know so that we can cite the reference in this datasheet.

    ab183825 has been referenced in 1 publication.

    • Blackwood CA  et al. Molecular Adaptations in the Rat Dorsal Striatum and Hippocampus Following Abstinence-Induced Incubation of Drug Seeking After Escalated Oxycodone Self-Administration. Mol Neurobiol 56:3603-3615 (2019). PubMed: 30155791

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