Key features and details
- Rabbit polyclonal to KAR
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-KAR antibody
DescriptionRabbit polyclonal to KAR
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Cynomolgus monkey
- WB: U-251 MG and A431 whole cell lysates. IHC-P: Human breast cancer and small intestine tissues. ICC/IF: U-251 MG cells.
Previously labelled as HSD17B12.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236990 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 34 kDa.|
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/20 - 1/200.|
FunctionCatalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation. Its strong expression in ovary and mammary gland suggest that it may constitute the major enzyme responsible for the conversion of E1 to E2 in women. Also has 3-ketoacyl-CoA reductase activity, reducing both long chain 3-ketoacyl-CoAs and long chain fatty acyl-CoAs, suggesting a role in long fatty acid elongation.
Tissue specificityExpressed in most tissues tested. Highly expressed in the ovary and mammary. Expressed in platelets.
PathwaySteroid biosynthesis; estrogen biosynthesis.
Lipid metabolism; fatty acid biosynthesis.
Sequence similaritiesBelongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.
DomainThe di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins.
Cellular localizationEndoplasmic reticulum membrane.
- Information by UniProt
- 12,3-ketoacyl-CoA reductase,short chain antibody
- 17 beta HSD 12 antibody
- 17 beta hydroxysteroid dehydrogenase 12 antibody
All lanes : Anti-KAR antibody (ab236990) at 1/500 dilution
Lane 1 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 34 kDa
Paraffin-embedded human breast cancer tissue stained for KAR using ab236990 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human small intestine tissue stained for KAR using ab236990 at 1/100 dilution in immunohistochemical analysis.
U-251 MG (human brain glioma cell line) cells labeling KAR using ab236990 (green) at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236990 has not yet been referenced specifically in any publications.