Anti-IPO5 antibody (ab187175)
Key features and details
- Rabbit polyclonal to IPO5
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-IPO5 antibody
See all IPO5 primary antibodies -
Description
Rabbit polyclonal to IPO5 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human IPO5 aa 20-300.
Database link: O00410 -
Positive control
- SW620, Hela, 293T, HepG2, mouse skeletal muscle, mouse testis, mouse heart, mouse brain and mouse ovary extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Constituents: 49% PBS, 50% Glycerol, 0.02% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
1/500 - 1/2000. Predicted molecular weight: 124 kDa.
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IHC-P |
1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/500 - 1/2000. Predicted molecular weight: 124 kDa. |
IHC-P
1/50 - 1/200. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. -
Sequence similarities
Belongs to the importin beta family.
Contains 6 HEAT repeats.
Contains 1 importin N-terminal domain. -
Cellular localization
Cytoplasm. Nucleus. Nucleus > nucleolus. Nucleus; nuclear rim. Found particularly in the nuclear rim and nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 3843 Human
- Entrez Gene: 70572 Mouse
- Entrez Gene: 306182 Rat
- Omim: 602008 Human
- SwissProt: O00410 Human
- SwissProt: Q8BKC5 Mouse
- Unigene: 712598 Human
- Unigene: 221452 Mouse
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Alternative names
- DKFZp686O1576 antibody
- FLJ43041 antibody
- IMB3 antibody
see all
Images
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All lanes : Anti-IPO5 antibody (ab187175) at 1/500 dilution
Lane 1 : SW620 cell extract
Lane 2 : Hela cell extract
Lane 3 : 293T cell extract
Lane 4 : HepG2 cell extract
Lane 5 : Mouse skeletal muscle extract
Lane 6 : Mouse testis extract
Lane 7 : Mouse heart extract
Lane 8 : Mouse brain extract
Lane 9 : Mouse ovary extract
Predicted band size: 124 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue labelling Karyopherin beta 3 with ab187175 at 1/200. Magnification: 400x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue labelling Karyopherin beta 3 with ab187175 at 1/200. Magnification: 400x.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue labelling Karyopherin beta 3 with ab187175 at 1/200. Magnification: 400x.
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Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab187175. Blue DAPI for nuclear staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab187175 has been referenced in 1 publication.
- Li D et al. DDX56 inhibits type I interferon by disrupting assembly of IRF3-IPO5 to inhibit IRF3 nucleus import. J Cell Sci 133:N/A (2019). PubMed: 31340999