For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    kat1--hat1-antibody-epr18775-ab194296.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
Share by email
Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)

  • Datasheet
  • Certificate of Compliance
Reviews (1) Submit a question References (10)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
  • Western blot - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
  • Western blot - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
  • Western blot - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
  • Immunocytochemistry/ Immunofluorescence - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
  • Immunocytochemistry/ Immunofluorescence - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
  • Flow Cytometry (Intracellular) - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
  • Immunoprecipitation - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
  • Immunoprecipitation - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
  • Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18775] to KAT1 / HAT1
  • Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

You may also be interested in

ELISA
Product image
Human MMP2 ELISA Kit (ab100606)
Biochemical
Product image
Dithiothreitol (DTT), reducing reagent (ab141390)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-KAT1 / HAT1 antibody [EPR18775]
    See all KAT1 / HAT1 primary antibodies
  • Description

    Rabbit monoclonal [EPR18775] to KAT1 / HAT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, MCF7, F9, LLC1, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human fetal liver and fetal kidney lysates; Mouse thymus lysate; Mouse and rat colon, brain, kidney and spleen lysates. IHC-P: Human tonsil, Human gastric adenocarcinoma, mouse colon and rat colon tissues. ICC/IF: HeLa and F9 cells. Flow Cyt (intra): HeLa cells. IP: HeLa and F9 whole cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR18775
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HAT

Associated products

  • Alternative Versions

    • Anti-KAT1 / HAT1 antibody [EPR18775] - BSA and Azide free (ab251185)
    • Alexa Fluor® 488 Anti-KAT1 / HAT1 antibody [EPR18775] (ab252100)
    • Alexa Fluor® 647 Anti-KAT1 / HAT1 antibody [EPR18775] (ab252101)
  • Assay kits

    • Histone Acetyltransferase Inhibitor Screening Assay Kit (ab133099)
    • Histone Acetyltransferase Activity Assay Kit (Fluorometric) (ab204709)
    • Histone Acetyltransferase Activity Assay Kit (Colorimetric) (ab65352)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human KAT1 / HAT1 protein (ab93687)
  • Related Products

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab194296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/150.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa).
ICC/IF
1/500.
IP
1/50.
Notes
Flow Cyt (Intra)
1/150.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 49 kDa).
ICC/IF
1/500.
IP
1/50.

Target

  • Function

    Acetylates soluble but not nucleosomal histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, acetylates histone H2A at 'Lys-5' (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage.
  • Sequence similarities

    Belongs to the HAT1 family.
  • Developmental stage

    Highly expressed in mitotic cells (at protein level).
  • Cellular localization

    Nucleus matrix and Cytoplasm. Nucleus. Nucleus matrix. Nucleus > nucleoplasm. Localization is predominantly nuclear in normal cells. Treatment with hydrogen peroxide or ionizing radiation enhances nuclear localization through redistribution of existing protein.
  • Target information above from: UniProt accession O14929 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8520 Human
    • Entrez Gene: 107435 Mouse
    • Entrez Gene: 296501 Rat
    • Omim: 603053 Human
    • SwissProt: O14929 Human
    • SwissProt: Q8BY71 Mouse
    • SwissProt: Q5M939 Rat
    • Unigene: 632532 Human
    • Unigene: 272472 Mouse
    • Unigene: 139751 Rat
    see all
  • Alternative names

    • HAT 1 antibody
    • hat1 antibody
    • HAT1_HUMAN antibody
    • Histidine aminotransferase 1 antibody
    • Histone acetyltransferase 1 antibody
    • Histone acetyltransferase 1 antibody
    • Histone acetyltransferase type B catalytic subunit antibody
    • KAT1 antibody
    see all

Images

  • Western blot - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
    Western blot - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)

    Lane 1: Wild-type HAP1 cell lysate (40 µg)
    Lane 2: KAT1/HAT1 knockout HAP1 cell lysate (40 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4:Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab194296 observed at 48 kDa. Red - loading control, ab18058, observed at 37 kDa.

    ab194296 was shown to recognize KAT1/HAT1 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when KAT1/HAT1 knockout samples were examined. Wild-type and KAT1 / HAT1 knockout samples were subjected to SDS-PAGE. Ab194296 and ab18058 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4oC. Blots were developed with IRDye® 800CW Goat anti-Rabbit IgG (H + L) and IRDye® 680 Goat anti-Mouse IgG (H + L) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
    Western blot - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
    All lanes : Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 49 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
    Western blot - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
    All lanes : Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296) at 1/1000 dilution

    Lane 1 : Mouse colon lysate
    Lane 2 : Rat colon lysate
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 4 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
    Lane 5 : F9 (Mouse embyro testicular cancer cell line) whole cell lysate
    Lane 6 : LLC1 (Mouse lung carcinoma cell line) whole cell lysate
    Lane 7 : Mouse thymus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 49 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1 and 2: 3 minutes; Lane 3 and 4: 2 seconds; Lane 5, 6 and 7: 5 seconds

  • Western blot - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
    Western blot - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
    All lanes : Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Rat kidney lysate
    Lane 6 : Rat spleen lysate
    Lane 7 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 8 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 9 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 10 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 49 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1, 2, 4, 5 and 6: 3 minutes; Lane 3: 1 minute; Lane 7, 8, 9 and 10: 5 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling KAT1 / HAT1 with ab194296 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on the germinal center of Human tonsil is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)

    Immunohistochemical analysis of paraffin-embedded Human gastric adenocarcinoma tissue labeling KAT1 / HAT1 with ab194296 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on cancer cells of Human gastric adenocarcinoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling KAT1 / HAT1 with ab194296 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on epithelial cells of mouse colon is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling KAT1 / HAT1 with ab194296 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on epithelial cells of rat colon is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
    Immunocytochemistry/ Immunofluorescence - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling KAT1 / HAT1 with ab194296 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab194296 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
    Immunocytochemistry/ Immunofluorescence - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized F9 (Mouse embyro testicular cancer cell line) cells labeling KAT1 / HAT1 with ab194296 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on F9 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab194296 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
    Flow Cytometry (Intracellular) - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling KAT1 / HAT1 with ab194296 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody. 

     

     

  • Immunoprecipitation - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
    Immunoprecipitation - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)

    KAT1 / HAT1 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab194296 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab194296 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HeLa whole cell lysate 10ug (Input). 

    Lane 2: ab194296 IP in HeLa whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab194296 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

  • Immunoprecipitation - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
    Immunoprecipitation - Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)

    KAT1 / HAT1 was immunoprecipitated from 1mg of F9 (Mouse embyro testicular cancer cell line) whole cell lysate with ab194296 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab194296 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: F9 whole cell lysate 10ug (Input). 

    Lane 2: ab194296 IP in F9 whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab194296 in F9 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

  • Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)
    Anti-KAT1 / HAT1 antibody [EPR18775] (ab194296)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (10)

Publishing research using ab194296? Please let us know so that we can cite the reference in this datasheet.

ab194296 has been referenced in 10 publications.

  • Zhang HL  et al. SRC3 acetylates calmodulin in the mouse brain to regulate synaptic plasticity and fear learning. J Biol Chem 297:101044 (2021). PubMed: 34358562
  • Toniyan KA  et al. Organization of the Cytoskeleton in Ectopic Foci of the Endometrium with Rare Localization. Biomedicines 9:N/A (2021). PubMed: 34440202
  • Fan P  et al. Overexpressed histone acetyltransferase 1 regulates cancer immunity by increasing programmed death-ligand 1 expression in pancreatic cancer. J Exp Clin Cancer Res 38:47 (2019). PubMed: 30709380
  • Gruber JJ  et al. HAT1 Coordinates Histone Production and Acetylation via H4 Promoter Binding. Mol Cell 75:711-724.e5 (2019). PubMed: 31278053
  • Loktev SS & Ogneva IV DNA Methylation of Mouse Testes, Cardiac and Lung Tissue During Long-Term Microgravity Simulation. Sci Rep 9:7974 (2019). PubMed: 31138883
  • Gruber JJ  et al. Chromatin Remodeling in Response to BRCA2-Crisis. Cell Rep 28:2182-2193.e6 (2019). PubMed: 31433991
  • Yang G  et al. HAT1 signaling confers to assembly and epigenetic regulation of HBV cccDNA minichromosome. Theranostics 9:7345-7358 (2019). PubMed: 31695772
  • Xia P  et al. MicroRNA-377 exerts a potent suppressive role in osteosarcoma through the involvement of the histone acetyltransferase 1-mediated Wnt axis. J Cell Physiol 234:22787-22798 (2019). PubMed: 31152456
  • Hunt LC  et al. A Key Role for the Ubiquitin Ligase UBR4 in Myofiber Hypertrophy in Drosophila and Mice. Cell Rep 28:1268-1281.e6 (2019). PubMed: 31365869
  • Aksu M  et al. Xpo7 is a broad-spectrum exportin and a nuclear import receptor. J Cell Biol 217:2329-2340 (2018). PubMed: 29748336

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

ChIP abreview for Anti-KAT1 / HAT1 antibody [EPR18775]

Below Average
Abreviews
Abreviews
abreview image
Application
ChIP
Sample
Human Cell lysate - nuclear (K562)
Specification
K562
Detection step
Other
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Read More

Mr. Andrew Perez

Verified customer

Submitted Mar 24 2022

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.