The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.
Involvement in disease
Note=A chromosomal aberration involving NCOA1 is a cause of rhabdomyosarcoma. Translocation t(2;2)(q35;p23) with PAX3 generates the NCOA1-PAX3 oncogene consisting of the N-terminus part of PAX3 and the C-terminus part of NCOA1. The fusion protein acts as a transcriptional activator. Rhabdomyosarcoma is the most common soft tissue carcinoma in childhood, representing 5-8% of all malignancies in children.
Belongs to the SRC/p160 nuclear receptor coactivator family. Contains 1 basic helix-loop-helix (bHLH) domain. Contains 1 PAS (PER-ARNT-SIM) domain.
The C-terminal (1107-1441) part mediates the histone acetyltransferase (HAT) activity. Contains 7 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. LXXLL motifs 3, 4 and 5 are essential for the association with nuclear receptors. LXXLL motif 7, which is not present in isoform 2, increases the affinity for steroid receptors in vitro.
Sumoylated; sumoylation increases its interaction with PGR and prolongs its retention in the nucleus. It does not prevent its ubiquitination and does not exert a clear effect on the stability of the protein. Ubiquitinated; leading to proteasome-mediated degradation. Ubiquitination and sumoylation take place at different sites. Phosphorylated upon DNA damage, probably by ATM or ATR.
Class E basic helix-loop-helix protein 74 antibody
F SRC 1 antibody
Hin 2 protein antibody
Hin2 protein antibody
mNRC 1 antibody
NCoA 1 antibody
Nuclear receptor coactivator 1 antibody
Nuclear receptor coactivator protein 1 antibody
NY REN 52 antigen antibody
Protein Hin 2 antibody
Protein Hin-2 antibody
Protein Hin2 antibody
Renal carcinoma antigen NY REN 52 antibody
Renal carcinoma antigen NY-REN-52 antibody
RIP 160 antibody
SRC 1 antibody
Steroid receptor coactivator 1 antibody
Immunoprecipitation - Anti-KAT13A / SRC1 antibody (ab10308)This image is a courtesy of Anonymous Abreview
ab10308 Immunoprecipitate KAT13A / SRC1 in human Huh-7 whole cell lysate. 1,000,000 cells were lysed and incubated with primary antibody at 4µg/mg lysate and Protein A matrix for 20 hours at 4°C. For western blotting an undiluted Abcam`s ab2859, Rabbit polyclonal to KAT13A / SRC1 was used.
Immunoprecipitation - Anti-KAT13A / SRC1 antibody (ab10308)Image courtesy of an anonymous Abreview.
ab10308 used in Immunoprecipitation. Total protein input is 1 x 106 cells.Whole cell lysate was prepared from VERO cells expressing recombinant SRC1 protein. Protein A matrix was used for the Immunoprecipitation step.ab10308 used a 4µg/mg lysate for 16 hours at 4°C. ab2859 was then used for Western Blot at 1µg/mg lysate.