Anti-KAT13A / SRC1 antibody (ab10308)
Key features and details
- Rabbit polyclonal to KAT13A / SRC1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KAT13A / SRC1 antibody
See all KAT13A / SRC1 primary antibodies -
Description
Rabbit polyclonal to KAT13A / SRC1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Horse, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Elephant -
Immunogen
Synthetic peptide within Human KAT13A/ SRC1 aa 1400-1500 (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q15788 -
Positive control
- Nuclear extract from HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance of 1.4 at 280 nm equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 156 kDa.
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IP | (3) |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 156 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3. -
Tissue specificity
Widely expressed. -
Involvement in disease
Note=A chromosomal aberration involving NCOA1 is a cause of rhabdomyosarcoma. Translocation t(2;2)(q35;p23) with PAX3 generates the NCOA1-PAX3 oncogene consisting of the N-terminus part of PAX3 and the C-terminus part of NCOA1. The fusion protein acts as a transcriptional activator. Rhabdomyosarcoma is the most common soft tissue carcinoma in childhood, representing 5-8% of all malignancies in children. -
Sequence similarities
Belongs to the SRC/p160 nuclear receptor coactivator family.
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAS (PER-ARNT-SIM) domain. -
Domain
The C-terminal (1107-1441) part mediates the histone acetyltransferase (HAT) activity.
Contains 7 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. LXXLL motifs 3, 4 and 5 are essential for the association with nuclear receptors. LXXLL motif 7, which is not present in isoform 2, increases the affinity for steroid receptors in vitro. -
Post-translational
modificationsSumoylated; sumoylation increases its interaction with PGR and prolongs its retention in the nucleus. It does not prevent its ubiquitination and does not exert a clear effect on the stability of the protein.
Ubiquitinated; leading to proteasome-mediated degradation. Ubiquitination and sumoylation take place at different sites.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8648 Human
- Entrez Gene: 313929 Rat
- Omim: 602691 Human
- SwissProt: Q15788 Human
- Unigene: 596314 Human
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Alternative names
- bHLHe74 antibody
- Class E basic helix-loop-helix protein 74 antibody
- F SRC 1 antibody
see all
Images
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Detection of KAT13A / SRC1 by western blot of immunoprecipitates.
Samples: Lane 1 and 2: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells prepared using NETN lysis buffer. Lane 3: Control IgG.
Antibodies: ab10308 (lot 2, lane 2) used for IP at 6 µg per reaction. KAT13A / SRC1 was also immunoprecipitated by a previous lot of this antibody (lot 1, lane 1). For blotting immunoprecipitated KAT13A / SRC1, ab10308 was used at 0.4 µg/mL.
Detection: Chemiluminescence with an exposure time of 30 seconds. -
All lanes : Anti-KAT13A / SRC1 antibody (ab10308) at 0.1 µg/ml
Lane 1 : HEK293T whole cell lysate at 50 µg
Lane 2 : HEK293T whole cell lysate at 15 µg
Lane 3 : HEK293T whole cell lysate at 5 µg
Lane 4 : NIH/3T3 whole cell lysate at 50 µg
Predicted band size: 156 kDa
Exposure time: 30 seconds
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab10308 has been referenced in 1 publication.
- Cho YJ et al. Bufalin suppresses endometriosis progression by inducing pyroptosis and apoptosis. J Endocrinol 237:255-269 (2018). PubMed: 29636364