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Cell Biology Apoptosis Nucleus Other
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Anti-KAT13C / NCOA2 antibody (ab2842)

  • Datasheet
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Immunocytochemistry/ Immunofluorescence - Anti-KAT13C / NCOA2 antibody (ab2842)
  • Immunocytochemistry/ Immunofluorescence - Anti-KAT13C / NCOA2 antibody (ab2842)

Key features and details

  • Rabbit polyclonal to KAT13C / NCOA2
  • Suitable for: ICC/IF
  • Reacts with: Human, Chinese hamster
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-KAT13C / NCOA2 antibody
    See all KAT13C / NCOA2 primary antibodies
  • Description

    Rabbit polyclonal to KAT13C / NCOA2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    Chinese hamster
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human KAT13C/ NCOA2 aa 206-217.
    Sequence:

    PLPDSEEEGHDS


    (Peptide available as ab4917)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

     

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand-dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcription activity of the nuclear receptor with which they associate. Glucocorticoid receptor interacting protein-1 (GRIP 1), or Transcription Intermediary Factor 2 (TIF 2), is a member of a family of transcriptional coactivator proteins which includes steroid receptor coactivator-1 (SRC 1) and Amplified in breast cancer-1 (AIB 1). GRIP1 has been shown to interact and stimulate transcriptional activity of the retinoic acid, retinoid X, vitamin D, mineralocorticoid, glucocorticoid, thyroid hormone, androgen, estrogen, progesterone, and peroxisome proliferator activated receptors. GRIP 1 also contains several copies of the conserved LXXLL/LLXXL motif which has been demonstrated to be critical to receptor-coactivator interactions.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Nucleus
    • Other
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Mediator Complex
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Types of disease
    • Obesity
    • Metabolism
    • Types of disease
    • Cancer
    • Cancer
    • Cell Death
    • Apoptosis
    • Nucleus
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • KAT13C / NCOA2 peptide (ab4917)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2842 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
Chinese hamster
Application Abreviews Notes
ICC/IF
Use a concentration of 1 - 2 µg/ml.
Notes
ICC/IF
Use a concentration of 1 - 2 µg/ml.

Target

  • Function

    Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues.
  • Involvement in disease

    Note=Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias. Inversion inv(8)(p11;q13) generates the KAT6A-NCOA2 oncogene, which consists of the N-terminal part of KAT6A and the C-terminal part of NCOA2/TIF2. KAT6A-NCOA2 binds to CREBBP and disrupts its function in transcription activation.
  • Sequence similarities

    Belongs to the SRC/p160 nuclear receptor coactivator family.
    Contains 1 bHLH (basic helix-loop-helix) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • Domain

    Contains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant.
    The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding.
    Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q15596 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10499 Human
    • Omim: 601993 Human
    • SwissProt: Q15596 Human
    • Unigene: 446678 Human
    • Alternative names

      • bHLHe75 antibody
      • Class E basic helix-loop-helix protein 75 antibody
      • glucocorticoid receptor interacting protein 1 antibody
      • GRIP 1 antibody
      • GRIP1 antibody
      • hTIF2 antibody
      • MED1 antibody
      • MGC138808 antibody
      • NCoA 2 antibody
      • NCoA-2 antibody
      • Ncoa2 antibody
      • NCOA2_HUMAN antibody
      • Nuclear receptor coactivator 2 antibody
      • Oncogene FGR antibody
      • p160 antibody
      • SRC 2 antibody
      • src2 antibody
      • Steroid receptor coactivator 2 antibody
      • TIF 2 antibody
      • TIF2 antibody
      • Transcriptional intermediary factor 2 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-KAT13C / NCOA2 antibody (ab2842)
      Immunocytochemistry/ Immunofluorescence - Anti-KAT13C / NCOA2 antibody (ab2842)

      Immunofluorescent analysis of PC-3 (Human prostate adenocarcinoma cell line) cells on 70% confluent log phase labeling KAT13C / NCOA2. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab2842 at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (1/300 dilution). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

       
    • Immunocytochemistry/ Immunofluorescence - Anti-KAT13C / NCOA2 antibody (ab2842)
      Immunocytochemistry/ Immunofluorescence - Anti-KAT13C / NCOA2 antibody (ab2842)

      Immunolocalization of KAT13C/NCOA2 in CHO (Chinese hamster ovary cell line) cells using ab2842.

    Protocols

    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab2842? Please let us know so that we can cite the reference in this datasheet.

    ab2842 has not yet been referenced specifically in any publications.

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