Overview

  • Product name

    Anti-KAT13C / NCOA2 antibody - C-terminal
    See all KAT13C / NCOA2 primary antibodies
  • Description

    Rabbit polyclonal to KAT13C / NCOA2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Pig
  • Immunogen

    Recombinant fragment within Human KAT13C/ NCOA2 (C terminal). The exact sequence is proprietary.
    Database link: Q15596

  • Positive control

    • ICC/IF: HeLa cells. WB: HeLa nuclear lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab227193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 159 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues.
  • Involvement in disease

    Note=Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias. Inversion inv(8)(p11;q13) generates the KAT6A-NCOA2 oncogene, which consists of the N-terminal part of KAT6A and the C-terminal part of NCOA2/TIF2. KAT6A-NCOA2 binds to CREBBP and disrupts its function in transcription activation.
  • Sequence similarities

    Belongs to the SRC/p160 nuclear receptor coactivator family.
    Contains 1 bHLH (basic helix-loop-helix) domain.
    Contains 1 PAS (PER-ARNT-SIM) domain.
  • Domain

    Contains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant.
    The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding.
    Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • bHLHe75 antibody
    • Class E basic helix-loop-helix protein 75 antibody
    • glucocorticoid receptor interacting protein 1 antibody
    • GRIP 1 antibody
    • GRIP1 antibody
    • hTIF2 antibody
    • MED1 antibody
    • MGC138808 antibody
    • NCoA 2 antibody
    • NCoA-2 antibody
    • Ncoa2 antibody
    • NCOA2_HUMAN antibody
    • Nuclear receptor coactivator 2 antibody
    • Oncogene FGR antibody
    • p160 antibody
    • SRC 2 antibody
    • src2 antibody
    • Steroid receptor coactivator 2 antibody
    • TIF 2 antibody
    • TIF2 antibody
    • Transcriptional intermediary factor 2 antibody
    see all

Images

  • Anti-KAT13C / NCOA2 antibody - C-terminal (ab227193) at 1/500 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate at 20 µg

    Secondary
    HRP-conjugated anti-rabbit IgG

    Developed using the ECL technique.

    Predicted band size: 159 kDa



    5% SDS PAGE

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for KAT13C / NCOA2 (green) using ab227193 (1/500 dilution) in ICC/IF.

    Counterstain: Alpha-tubulin filaments were labeled with an anti-alpha-Tubulin antibody (red).

References

ab227193 has not yet been referenced specifically in any publications.

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