Product nameAnti-KAT13C / NCOA2 antibody [2615C1a]
See all KAT13C / NCOA2 primary antibodies
DescriptionMouse monoclonal [2615C1a] to KAT13C / NCOA2
Tested applicationsSuitable for: WB, Dot blotmore details
Species reactivityReacts with: Human
Recombinant fragment of NCOA2 (Human)
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (pproximately 95%) fetal bovine serum
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab53544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Predicted molecular weight: 159 kDa.|
|Dot blot||Use at an assay dependent dilution.|
FunctionTranscriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues.
Involvement in diseaseNote=Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias. Inversion inv(8)(p11;q13) generates the KAT6A-NCOA2 oncogene, which consists of the N-terminal part of KAT6A and the C-terminal part of NCOA2/TIF2. KAT6A-NCOA2 binds to CREBBP and disrupts its function in transcription activation.
Sequence similaritiesBelongs to the SRC/p160 nuclear receptor coactivator family.
Contains 1 bHLH (basic helix-loop-helix) domain.
Contains 1 PAS (PER-ARNT-SIM) domain.
DomainContains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant.
The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding.
Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- bHLHe75 antibody
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Anti-KAT13C / NCOA2 antibody [2615C1a] (ab53544) + Immunized recombinant protein
Predicted band size: 159 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein as the immunizing recombinant fragment protein was used as the test antigen