For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    kat13c-ncoa2-antibody-ab10491.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Nuclear Signaling Pathways Nuclear Receptors Co-activators/co-repressors
Share by email

Anti-KAT13C / NCOA2 antibody (ab10491)

  • Datasheet
  • SDS
Reviews (4)Q&A (1)References (8)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-KAT13C / NCOA2 antibody (ab10491)

    Key features and details

    • Rabbit polyclonal to KAT13C / NCOA2
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Primary
    Product image
    Anti-JunB antibody [EPR6518] (ab128878)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Primary
    Product image
    Anti-TBL1 antibody (ab24548)

    View more associated products

    Overview

    • Product name

      Anti-KAT13C / NCOA2 antibody
      See all KAT13C / NCOA2 primary antibodies
    • Description

      Rabbit polyclonal to KAT13C / NCOA2
    • Host species

      Rabbit
    • Specificity

      We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Rat KAT13C/ NCOA2 aa 700-750.
      Database link: Q15596

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7
      Preservative: 0.1% Sodium azide
      Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Antibodies were affinity purified using the peptide immobilized on solid support.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • Nuclear Receptors
      • Co-activators/co-repressors
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Other factors
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Mediator Complex
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of lipids and lipoproteins
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipid metabolism
      • Metabolism
      • Types of disease
      • Obesity
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab10491 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Rat
    Rabbit
    Guinea pig
    Cow
    Dog
    Pig
    Chimpanzee
    Ferret
    Gorilla
    Chinese hamster
    Application Abreviews Notes
    WB (3)
    Notes
  • Application notes
    IP: Use at a concentration of 1 - 4 µg/mg of lysate.
    WB: 1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 175 kDa).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues.
    • Involvement in disease

      Note=Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias. Inversion inv(8)(p11;q13) generates the KAT6A-NCOA2 oncogene, which consists of the N-terminal part of KAT6A and the C-terminal part of NCOA2/TIF2. KAT6A-NCOA2 binds to CREBBP and disrupts its function in transcription activation.
    • Sequence similarities

      Belongs to the SRC/p160 nuclear receptor coactivator family.
      Contains 1 bHLH (basic helix-loop-helix) domain.
      Contains 1 PAS (PER-ARNT-SIM) domain.
    • Domain

      Contains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant.
      The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding.
      Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently.
    • Post-translational
      modifications

      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q15596 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 10499 Human
      • Entrez Gene: 83724 Rat
      • Omim: 601993 Human
      • SwissProt: Q15596 Human
      • SwissProt: Q9WUI9 Rat
      • Unigene: 446678 Human
      • Unigene: 49136 Rat
      • Alternative names

        • bHLHe75 antibody
        • Class E basic helix-loop-helix protein 75 antibody
        • glucocorticoid receptor interacting protein 1 antibody
        • GRIP 1 antibody
        • GRIP1 antibody
        • hTIF2 antibody
        • MED1 antibody
        • MGC138808 antibody
        • NCoA 2 antibody
        • NCoA-2 antibody
        • Ncoa2 antibody
        • NCOA2_HUMAN antibody
        • Nuclear receptor coactivator 2 antibody
        • Oncogene FGR antibody
        • p160 antibody
        • SRC 2 antibody
        • src2 antibody
        • Steroid receptor coactivator 2 antibody
        • TIF 2 antibody
        • TIF2 antibody
        • Transcriptional intermediary factor 2 antibody
        see all

      Images

      • Western blot - Anti-KAT13C / NCOA2 antibody (ab10491)
        Western blot - Anti-KAT13C / NCOA2 antibody (ab10491)

        ab10491 detection of human NCOA2 (SRC2) by Western blot and immunoprecipitation. Samples: Nuclear extract (5 µg for WB; 10 mg for Co-IP) from HeLa cells. Antibody: ab10491used at 0.33 µg/ml (A) for WB and 20 µg/10 mg lysate (B) for IP.  Detection: Alkaline phosphatase conjugated goat anti-rabbit IgG diluted 1/3000 with NBT-BCIP as substrate for 20 min. (A) or Coomassie Brilliant Blue R250 staining of SDS-PAGE gel followed by mass spectrometry to confirm the identity of the band that is NCOA2 (SRC2) (B).  

        ab10491 detection of human NCOA2 (SRC2) by Western blot and immunoprecipitation. Samples: Nuclear extract (5 µg for WB; 10 mg for Co-IP) from HeLa cells. Antibody: ab10491used at 0.33 µg/ml (A) f

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (8)

      Publishing research using ab10491? Please let us know so that we can cite the reference in this datasheet.

      ab10491 has been referenced in 8 publications.

      • Rollins DA  et al. Glucocorticoid-induced phosphorylation by CDK9 modulates the coactivator functions of transcriptional cofactor GRIP1 in macrophages. Nat Commun 8:1739 (2017). PubMed: 29170386
      • Takeda K  et al. Corepressive function of nuclear receptor coactivator 2 in androgen receptor of prostate cancer cells treated with antiandrogen. BMC Cancer 16:332 (2016). PubMed: 27225190
      • Coppo M  et al. The transcriptional coregulator GRIP1 controls macrophage polarization and metabolic homeostasis. Nat Commun 7:12254 (2016). WB, ChIP, ICC/IF ; Mouse . PubMed: 27464507
      • Wagner M  et al. NCOA3 is a selective co-activator of estrogen receptor a-mediated transactivation of PLAC1 in MCF-7 breast cancer cells. BMC Cancer 13:570 (2013). WB ; Human . PubMed: 24304549
      • Lit LC  et al. LATS2 is a modulator of estrogen receptor alpha. Anticancer Res 33:53-63 (2013). WB, ICC/IF ; Human . PubMed: 23267128
      • Amazit L  et al. Ligand-Dependent Degradation of SRC-1 Is Pivotal for Progesterone Receptor Transcriptional Activity. Mol Endocrinol 25:394-408 (2011). WB ; Human . PubMed: 21273440
      • Jelcick AS  et al. Genetic variations strongly influence phenotypic outcome in the mouse retina. PLoS One 6:e21858 (2011). WB ; Mouse . PubMed: 21779340
      • Flammer JR  et al. The type I interferon signaling pathway is a target for glucocorticoid inhibition. Mol Cell Biol 30:4564-74 (2010). PubMed: 20679482

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-5 of 5 Abreviews or Q&A

      Western blot abreview for Anti-KAT13C / NCOA2 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      20 µg
      Gel Running Conditions
      Reduced Denaturing (7% Bis-Tris gels)
      Sample
      Human Cell lysate - whole cell (primary myometrial cells)
      Specification
      primary myometrial cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Dr. Claire Hudson

      Verified customer

      Submitted Dec 22 2014

      Immunoprecipitation abreview for Anti-KAT13C / NCOA2 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunoprecipitation
      Sample
      Human Cell lysate - whole cell (huh-7)
      Total protein in input
      1e+006 cells
      Specification
      huh-7
      Immuno-precipitation step
      Protein A
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 23 2010

      Western blot abreview for Anti-KAT13C / NCOA2 antibody

      Below Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (liver)
      Loading amount
      15 µg
      Specification
      liver
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 09 2010

      Western blot abreview for Anti-KAT13C / NCOA2 antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (huh-7 cells)
      Loading amount
      25000 cells
      Specification
      huh-7 cells
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 01 2008

      Question

      10491 and/or 10508 (both are NCOA2 antibodies). As I understand from your product datasheets immunogen was (3 prime end of exon 13 (and exon 26, for second ab). Can you please tell me which amino acids do these region(s) correspond to.

      Read More

      Abcam community

      Verified customer

      Asked on Jun 10 2004

      Answer

      The epitope recognized by ab10491 maps to a region between residues 750 and 800 of Swiss-Prot entry Q15596 (http://au.expasy.org/cgi-bin/niceprot.pl?Q15596). The epitope recognized by ab10508 maps to a region within the C-terminal 50 residues of Swiss-Prot entry Q15596 (http://au.expasy.org/cgi-bin/niceprot.pl?Q15596).

      Read More

      Abcam Scientific Support

      Answered on Jun 14 2004

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.