Anti-KAT13C / NCOA2 antibody (ab10491)
Key features and details
- Rabbit polyclonal to KAT13C / NCOA2
- Suitable for: WB, IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-KAT13C / NCOA2 antibody
See all KAT13C / NCOA2 primary antibodies -
Description
Rabbit polyclonal to KAT13C / NCOA2 -
Host species
Rabbit -
Specificity
We have data to indicate that this antibody may not cross react with Xenopus laevis. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Gorilla, Chinese hamster
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Immunogen
Synthetic peptide corresponding to Human KAT13C/ NCOA2 aa 700-800.
Database link: Q15596 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibodies were affinity purified using the peptide immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10491 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 175 kDa).
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| IHC-P |
Use at an assay dependent concentration.
|
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| IP | (1) |
Use 6µg for 10 cells.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 175 kDa). |
|
IHC-P
Use at an assay dependent concentration. |
|
IP
Use 6µg for 10 cells. |
Target
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Function
Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues. -
Involvement in disease
Note=Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias. Inversion inv(8)(p11;q13) generates the KAT6A-NCOA2 oncogene, which consists of the N-terminal part of KAT6A and the C-terminal part of NCOA2/TIF2. KAT6A-NCOA2 binds to CREBBP and disrupts its function in transcription activation. -
Sequence similarities
Belongs to the SRC/p160 nuclear receptor coactivator family.
Contains 1 bHLH (basic helix-loop-helix) domain.
Contains 1 PAS (PER-ARNT-SIM) domain. -
Domain
Contains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant.
The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding.
Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10499 Human
- Entrez Gene: 17978 Mouse
- Entrez Gene: 83724 Rat
- Omim: 601993 Human
- SwissProt: Q15596 Human
- SwissProt: Q61026 Mouse
- SwissProt: Q9WUI9 Rat
- Unigene: 446678 Human
see all -
Alternative names
- bHLHe75 antibody
- Class E basic helix-loop-helix protein 75 antibody
- glucocorticoid receptor interacting protein 1 antibody
see all
Images
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Immunoprecipitation - Anti-KAT13C / NCOA2 antibody (ab10491)
ab10491 used for WB at 0.1 µg/ml (A)
Lane 1: Whole cell lysate Hela, 50 ug
Lane 2: Whole cell lysate Hela, 15 ug
Lane 3: Whole cell lysate HEK293T, 50 ug
Lane 4: Whole cell lysate Jurkat, 50 ug
ab10491 was used at 1 µg/ml for WB and used for IP at 6 µg/mg lysate (B)
Lane 1-3: ab10491]
Lane4: Control IgG
Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
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Western blot - Anti-KAT13C / NCOA2 antibody (ab10491)All lanes : Anti-KAT13C / NCOA2 antibody (ab10491) at 1 µg/ml
Lane 1 : TCMK-1 cells
Lane 2 : 4T1 cells
Lane 3 : CT26.WT cells
Lysates/proteins at 50 µg per lane.
Predicted band size: 175 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (11)
ab10491 has been referenced in 11 publications.
- Mimouna S et al. Transcription cofactor GRIP1 differentially affects myeloid cell-driven neuroinflammation and response to IFN-ß therapy. J Exp Med 218:N/A (2021). PubMed: 33045064
- Yao C et al. Thevebioside, the active ingredient of traditional Chinese medicine, promotes ubiquitin-mediated SRC-3 degradation to induce NSCLC cells apoptosis. Cancer Lett 493:167-177 (2020). PubMed: 32829007
- Escoter-Torres L et al. Anti-inflammatory functions of the glucocorticoid receptor require DNA binding. Nucleic Acids Res 48:8393-8407 (2020). PubMed: 32619221
- Rollins DA et al. Glucocorticoid-induced phosphorylation by CDK9 modulates the coactivator functions of transcriptional cofactor GRIP1 in macrophages. Nat Commun 8:1739 (2017). PubMed: 29170386
- Takeda K et al. Corepressive function of nuclear receptor coactivator 2 in androgen receptor of prostate cancer cells treated with antiandrogen. BMC Cancer 16:332 (2016). PubMed: 27225190