For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
If you continue without changing your cookie settings, we'll assume you’re happy with this.
mouse heart samples
ChIP with demo, clock and isotype crtl
detection by RT-PCR
pos. crtl: no signal
clock: same signal like neg. crtl
demo: good signal
Ab: 1/50 , 1/5000 (10 ul Ab, 500 ul chromatin)
suggestion: try 1/500 or 1/1000 as well, check publication which mentioned ChIP
Asked on Aug 28 2012
Thank you for contacting us.
I am sorry to hear that the antibody has not been working as expected.
As we discussed over the phone, trying a dilution of 1/500 or 1/1000 might help to improve your results.
Please let me know if this suggestion is of help.
I look forward to hear back from you.
Use our products? Submit an Abreview. Earn rewards!
Answered on Aug 28 2012