Anti-KAT2A / GCN5 antibody - C-terminal (ab153903)
Key features and details
- Rabbit polyclonal to KAT2A / GCN5 - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-KAT2A / GCN5 antibody - C-terminal
See all KAT2A / GCN5 primary antibodies -
Description
Rabbit polyclonal to KAT2A / GCN5 - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB MouseHuman
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Immunogen
Recombinant fragment, corresponding to a region within C terminal amino acids 589-810 of Human KAT2A/ GCN5.
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Positive control
- IMR32, NIH3T3, JC and BCL-1 whole cell lysates; MCF7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab153903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| WB |
Mouse
Human
|
| All applications |
Rat
Chicken
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 94 kDa.
|
|
| ICC/IF |
1/100 - 1/1000.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 94 kDa. |
|
ICC/IF
1/100 - 1/1000. |
Target
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Function
Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Acetylation of histones gives a specific tag for epigenetic transcription activation. Has significant histone acetyltransferase activity with core histones, but not with nucleosome core particles. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. -
Tissue specificity
Expressed in all tissues tested, with most abundant expression in ovary. -
Sequence similarities
Belongs to the GCN5 family.
Contains 1 bromo domain.
Contains 1 N-acetyltransferase domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 374232 Chicken
- Entrez Gene: 2648 Human
- Entrez Gene: 14534 Mouse
- Entrez Gene: 303539 Rat
- Omim: 602301 Human
- SwissProt: Q92830 Human
- SwissProt: Q9JHD2 Mouse
- Unigene: 463045 Human
see all -
Alternative names
- 1110051E14Rik antibody
- AW212720 antibody
- EC 2.3.1.48 antibody
see all
Images
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Western blot - Anti-KAT2A / GCN5 antibody - C-terminal (ab153903)Anti-KAT2A / GCN5 antibody - C-terminal (ab153903) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg
Predicted band size: 94 kDa
7.5% SDS PAGE -
Western blot - Anti-KAT2A / GCN5 antibody - C-terminal (ab153903)All lanes : Anti-KAT2A / GCN5 antibody - C-terminal (ab153903) at 1/1000 dilution
Lane 1 : NIH3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL-1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 94 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-KAT2A / GCN5 antibody - C-terminal (ab153903)Immunofluorescent analysis of paraformaldehyde-fixed MCF7 cells labeling KAT2A / GCN5 with ab153903 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
Protocols
References (1)
ab153903 has been referenced in 1 publication.
- Torres-Zelada EF et al. The Drosophila Dbf4 ortholog Chiffon forms a complex with Gcn5 that is necessary for histone acetylation and viability. J Cell Sci 132:N/A (2019). PubMed: 30559249
