Anti-KAT2A / GCN5 antibody - C-terminal (ab153903)
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Overview
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Product name
Anti-KAT2A / GCN5 antibody - C-terminal
See all KAT2A / GCN5 primary antibodies -
Description
Rabbit polyclonal to KAT2A / GCN5 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken
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Immunogen
Recombinant fragment, corresponding to a region within C terminal amino acids 589-810 of Human KAT2A/ GCN5.
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Positive control
- IMR32, NIH3T3, JC and BCL-1 whole cell lysates; MCF7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 20% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab153903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 94 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Acetylation of histones gives a specific tag for epigenetic transcription activation. Has significant histone acetyltransferase activity with core histones, but not with nucleosome core particles. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. -
Tissue specificity
Expressed in all tissues tested, with most abundant expression in ovary. -
Sequence similarities
Belongs to the GCN5 family.
Contains 1 bromo domain.
Contains 1 N-acetyltransferase domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 374232 Chicken
- Entrez Gene: 2648 Human
- Entrez Gene: 14534 Mouse
- Entrez Gene: 303539 Rat
- Omim: 602301 Human
- SwissProt: Q92830 Human
- SwissProt: Q9JHD2 Mouse
- Unigene: 463045 Human
see all -
Alternative names
- 1110051E14Rik antibody
- AW212720 antibody
- EC 2.3.1.48 antibody
see all
Images
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Western blot - Anti-KAT2A / GCN5 antibody - C-terminal (ab153903)Anti-KAT2A / GCN5 antibody - C-terminal (ab153903) at 1/1000 dilution + IMR32 whole cell lysate at 30 µg
Predicted band size: 94 kDa
7.5% SDS PAGE -
Western blot - Anti-KAT2A / GCN5 antibody - C-terminal (ab153903)All lanes : Anti-KAT2A / GCN5 antibody - C-terminal (ab153903) at 1/1000 dilution
Lane 1 : NIH3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL-1 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 94 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-KAT2A / GCN5 antibody - C-terminal (ab153903)Immunofluorescent analysis of paraformaldehyde-fixed MCF7 cells labeling KAT2A / GCN5 with ab153903 at 1/500 dilution. Lower panel co-stained with Hoechst 33342.
References
ab153903 has not yet been referenced specifically in any publications.
