Product nameAnti-KAT2B / PCAF antibody [EPR2670(N)]
See all KAT2B / PCAF primary antibodies
DescriptionRabbit monoclonal [EPR2670(N)] to KAT2B / PCAF
Tested applicationsSuitable for: WB, Flow Cyt, IPmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human KAT2B/ PCAF aa 150-250. The exact sequence is proprietary.
- HeLa, HT-1080 and A431 cell lysates; Hela cells
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab176316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 93 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/10 - 1/100.|
FunctionFunctions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.
Tissue specificityUbiquitously expressed but most abundant in heart and skeletal muscle.
Sequence similaritiesBelongs to the GCN5 family.
Contains 1 bromo domain.
Contains 1 N-acetyltransferase domain.
DomainThe bromodomain mediates binding to HIV-1 Tat.
- Information by UniProt
- CAF antibody
- CREBBP associated factor antibody
- GCN5 antibody
All lanes : Anti-KAT2B / PCAF antibody [EPR2670(N)] (ab176316) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HT-1080 cell lysate
Lane 3 : A431 cell lysate
Predicted band size: 93 kDa
Western blot analysis on immunoprecipitation pellet from HT-1080 cell lysate labeling KAT2B / PCAF using ab176316 at a 1/10 dilution.
Flow cytometric analysis of permeabilized Hela cells labeling KAT2B / PCAF using ab176316 at a 1/10 dilution (red), or a rabbit IgG control (green).
This product has been referenced in:
- Katamune C et al. Mutation of the gene encoding the circadian clock component PERIOD2 in oncogenic cells confers chemoresistance by up-regulating the Aldh3a1 gene. J Biol Chem 294:547-558 (2019). Read more (PubMed: 30429219) »
- Yang F et al. Sulforaphane induces autophagy by inhibition of HDAC6-mediated PTEN activation in triple negative breast cancer cells. Life Sci 213:149-157 (2018). Read more (PubMed: 30352240) »