Overview

  • Product name

    Anti-KAT2B / PCAF antibody
    See all KAT2B / PCAF primary antibodies
  • Description

    Rabbit polyclonal to KAT2B / PCAF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide, corresponding to a region within the C terminal amino acids 767-832 of Human KAT2B/ PCAF.

  • Positive control

    • 293T, A431, H1299, HeLa S3, HepG2, MOLT4, Raji cells, HeLa cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab96510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 93 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.
  • Tissue specificity

    Ubiquitously expressed but most abundant in heart and skeletal muscle.
  • Sequence similarities

    Belongs to the GCN5 family.
    Contains 1 bromo domain.
    Contains 1 N-acetyltransferase domain.
  • Domain

    The bromodomain mediates binding to HIV-1 Tat.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CAF antibody
    • CREBBP associated factor antibody
    • GCN5 antibody
    • GCN5L antibody
    • GCN5L1 antibody
    • Histone acetylase PCAF antibody
    • Histone acetyltransferase KAT2B antibody
    • Histone acetyltransferase PCAF antibody
    • K(lysine) acetyltransferase 2B antibody
    • KAT2B antibody
    • KAT2B_HUMAN antibody
    • Lysine acetyltransferase 2B antibody
    • P antibody
    • P/CAF antibody
    • p300/CBP associated factor antibody
    • P300/CBP-associated factor antibody
    • Pcaf antibody
    see all

Images

  • Anti-KAT2B / PCAF antibody (ab96510) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 93 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96510 at 1:200 dilution. Image 2: Merged with DNA probe.

References

This product has been referenced in:

  • Shirakawa K  et al. Salicylate, diflunisal and their metabolites inhibit CBP/p300 and exhibit anticancer activity. Elife 5:N/A (2016). WB . Read more (PubMed: 27244239) »
  • Rabhi N  et al. KAT2B Is Required for Pancreatic Beta Cell Adaptation to Metabolic Stress by Controlling the Unfolded Protein Response. Cell Rep 15:1051-1061 (2016). Read more (PubMed: 27117420) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - whole (Rat brain bilateral dorsal hippocampus, bilateral)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
90 µg
Specification
Rat brain bilateral dorsal hippocampus, bilateral
Blocking step
Milk as blocking agent for 1 hour(s) and 15 minute(s) · Concentration: 5% · Temperature: 24°C

Krista Mitchnick

Verified customer

Submitted Jul 02 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up