Product nameKAT2B/pCAF Inhibitor Screening Assay Kit (Fluorometric)
Sample typeInhibitor compounds
Assay typeEnzyme activity
KAT2B/pCAF Inhibitor Screening Assay Kit (Fluorometric) (ab196991) is an assay where histone H3 peptide and Acetyl CoA are used as acetylation substrates. KAT2B/pCAF acetylates the Histone H3 peptide and generates Coenzyme A with a free thiol group (CoA-SH). CoA-SH reacts subsequently with a Thiol Detecting Probe in order to increase the amount of fluorescence in the solution, which can be measured at Ex/Em = 392/482 nm. In the presence of KAT2B/pCAF specific inhibitors, the enzymatic activity is reduced ot completely abolished resulting in decreased or total loss of fluorescence.
This assay kit is a simple, sensitive and rapid tool to screen potential pCAF inhibitors.
KTA2B (K-(lysine) acetyltransferase 2B, EC 220.127.116.11), also known as P300/CBP-associated factor (pCAF), possesses intrinsic histone acetyltransferase (HAT) activity and is able to acetylated both histone H3 and histone H4. KTA2B/pCAF contributes to transcriptional activation by acetylating chromatin and transcription factors through its HAT activity. Deregulated HAT activity plays an important role in various cancers.
KTA2B/pCAF inhibitors have potential applications in cancer therapy.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests Acetyl CoA 1 vial H3 Peptide 1 vial pCAF Assay Buffer 1 x 25ml pCAF Enzyme 1 x 40µl pCAF Inhibitor 1 x 10µl Thiol Detecting Probe 1 x 200µl
FunctionFunctions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Also acetylates non-histone proteins, such as ACLY. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.
Tissue specificityUbiquitously expressed but most abundant in heart and skeletal muscle.
Sequence similaritiesBelongs to the acetyltransferase family. GCN5 subfamily.
Contains 1 bromo domain.
Contains 1 N-acetyltransferase domain.
DomainThe bromodomain mediates binding to HIV-1 Tat.
- Information by UniProt
- Histone acetylase PCAF
- Histone acetyltransferase KAT2B
- Histone acetyltransferase PCAF
ab196991 has not yet been referenced specifically in any publications.