Anti-KAT3A / CBP antibody - ChIP Grade (ab2832)
- Datasheet
- References (37)
- Protocols
Overview
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Product nameAnti-KAT3A / CBP antibody - ChIP Grade
See all KAT3A / CBP primary antibodies -
DescriptionRabbit polyclonal to KAT3A / CBP - ChIP Grade
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ChIP, IHC-P, IP, ICC/IF, CHIPseq, Inhibition Assaymore details
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Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Non human primates
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Immunogen
Synthetic peptide corresponding to Human KAT3A/ CBP aa 162-176.
Sequence:ATSSPATSQTGPGIC
(Peptide available asab4916) -
Positive control
- HeLa cell lysate. IHC-P: human pancreas FFPE tissue sections
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
PurityImmunogen affinity purified
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Primary antibody notesCyclic AMP-responsive enhancer binding protein (CREB) binding protein (CBP) and p300 are closely related transcriptional coactivators that have been shown to directly interact with many different DNA-binding transcription factors including nuclear hormone receptors, CREB (cyclic AMP-responsive enhancer binding protein), c-Fos, c-Jun/v-Jun, c-Myb/v-Myb, TFIIB and MyoD.Both CBP and p300 have been shown to display histone acetyltransferase (HAT) activity, capable of acetylating all four core histone particles in nucleosomes.As a result of HAT activity, it has been suggested CBP and p300 may play a direct role in activating chromatin for transcription.Single point mutations in CBP have been proposed as causative factors in the developmental abnormalities of Rubinstein-Taybi syndrome (RTS).Although both CBP and p300 appear to function similarly, the inability of p300 to rescue CBP malfunction iRTS suggests intrinsic functional differences between CBP and p300.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab2832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 265 kDa (predicted molecular weight: 265 kDa).Can be blocked with Human KAT3A / CBP peptide (ab4916). | |
| ChIP | Use at an assay dependent concentration. PubMed: 20111703 Recommended to be used at 2-4µg for 1-2x106 cells. |
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| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IP | Use at an assay dependent concentration. PubMed: 20403362 | |
| ICC/IF | 1/100. | |
| CHIPseq | Use at an assay dependent concentration. PubMed: 22196736 | |
| Inhibition Assay | Use at an assay dependent concentration. |
Target
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FunctionAcetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 coactivator. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1 in the presence of EP300.
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Involvement in diseaseNote=Chromosomal aberrations involving CREBBP may be a cause of acute myeloid leukemias. Translocation t(8;16)(p11;p13) with MYST3/MOZ; translocation t(11;16)(q23;p13.3) with MLL/HRX; translocation t(10;16)(q22;p13) with MYST4/MORF. MYST3-CREBBP may induce leukemia by inhibiting RUNX1-mediated transcription.
Defects in CREBBP are a cause of Rubinstein-Taybi syndrome type 1 (RSTS1) [MIM:180849]. RSTS1 is an autosomal dominant disorder characterized by craniofacial abnormalities, broad thumbs, broad big toes, mental retardation and a propensity for development of malignancies. -
Sequence similaritiesContains 1 bromo domain.
Contains 1 KIX domain.
Contains 2 TAZ-type zinc fingers.
Contains 1 ZZ-type zinc finger. -
DomainThe KIX domain mediates binding to HIV-1 Tat.
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Post-translational
modificationsMethylation of the KIX domain by CARM1 blocks association with CREB. This results in the blockade of CREB signaling, and in activation of apoptotic response.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylation negatively regulates transcriptional activity via the recruitment of DAAX. -
Cellular localizationCytoplasm. Nucleus. Recruited to nuclear bodies by SS18L1/CREST. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus.
- Information by UniProt
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Database links
- Entrez Gene: 1387 Human
- Entrez Gene: 12914 Mouse
- Entrez Gene: 54244 Rat
- Omim: 600140 Human
- SwissProt: Q92793 Human
- SwissProt: P45481 Mouse
- SwissProt: Q6JHU9 Rat
- Unigene: 459759 Human
see all -
Alternative names
- CBP antibody
- CBP_HUMAN antibody
- CREB binding protein antibody
see all
Images
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ChIP - Anti-KAT3A / CBP antibody - ChIP Grade (ab2832)Sonicated chromatin prepared from U2OS cells was subjected to the ChIP procedure with ab2832 to CBP. Immunoprecipitated chromatin was analysed in the promoter region of c-FOS (active) and in exon 2 of MYO-D (inactive). Values are % of inputs. 2–4 µg of ab2832 and 1-2x106 cells were used in each ChIP experiment.
Sergei Denissov, Prof. Henk Stunnenberg lab, University Nijmengen, NL
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Immunocytochemistry/ Immunofluorescence - Anti-KAT3A / CBP antibody - ChIP Grade (ab2832)Immunofluorescent analysis of MCF-7 cells, labeling KAT3A/CBP with ab2832 (right) compared with a negative control without ab2832 (left). Cells were formalin fixed, permeabilized with 0.1% Triton X-100 for 5-10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with anti-KAT3A/CBP diluted 1/100 in 3% BSA/PBS overnight at 4°C. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with DAPI (blue). The nuclear localization of KAT3A/CBP can be observed (green).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT3A / CBP antibody - ChIP Grade (ab2832)IHC image of KAT3A/CBP staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2832, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunocytochemistry/ Immunofluorescence - Anti-KAT3A / CBP antibody - ChIP Grade (ab2832)Immunofluorescent analysis of HeLa cells, labeling KAT3A/CBP with ab2832 (right) compared with a negative control without ab2832 (left). Cells were formalin fixed, permeabilized with 0.1% Triton X-100 for 5-10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with anti-KAT3A/CBP diluted 1/100 in 3% BSA/PBS overnight at 4°C. Actin was stained using Alexa Fluor 554 (red) and nuclei were stained with DAPI (blue). The nuclear localization of KAT3A/CBP can be observed (green).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KAT3A / CBP antibody - ChIP Grade (ab2832)Immunohistochemical analysis of FFPE mouse colon tissue, labeling KAT3A/CBP with ab2832 (right) compared with a negative control without ab2832 (left). Heat antigen retrieval performed with 10mM Sodium Citrate (pH 6) for 8-15 minutes. Tissue blocked with 3% H2O2-methanol for 15 minutes at room temperature. Incubation with ab2832 diluted 1/2000 in 3% BSA-PBS overnight at 4°C. Counterstaining with hematoxylin.
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Immunocytochemistry/ Immunofluorescence - Anti-KAT3A / CBP antibody - ChIP Grade (ab2832)Immunofluorescent analysis of NIH-3T3 cells, labeling KAT3A/CBP with ab2832 (right) compared with a negative control without ab2832 (left). Cells were formalin fixed, permeabilized with 0.1% Triton X-100 for 5-10 minutes, and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with anti-KAT3A/CBP diluted 1/100 in 3% BSA/PBS overnight at 4°C. Nuclei were stained with DAPI (blue).
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Western blot - Anti-KAT3A / CBP antibody - ChIP Grade (ab2832)ab2832 using HeLa cell lysate. ab2832 using HeLa cell lysate.
Datasheets and documents
Protocols
References
This product has been referenced in:
- Singh S et al. Decreased level of histone acetylation in the infralimbic prefrontal cortex following immediate extinction may result in deficit of extinction memory. Brain Res Bull 140:355-364 (2018). Read more (PubMed: 29908895) »
- Gökyildirim MY et al. Targeting CREB-binding protein overrides LPS induced radioresistance in non-small cell lung cancer cell lines. Oncotarget 9:28976-28988 (2018). Read more (PubMed: 29989005) »
