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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Anti-KAT3B / p300 antibody [3G230 / NM-11] - BSA and Azide free (ab264432)

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ChIP - Anti-KAT3B / p300 antibody [3G230 / NM-11] - BSA and Azide free (ab264432)
  • Western blot - Anti-KAT3B / p300 antibody [3G230 / NM-11] - BSA and Azide free (ab264432)

Key features and details

  • Mouse monoclonal [3G230 / NM-11] to KAT3B / p300 - BSA and Azide free
  • Suitable for: WB, IP, ICC/IF, ChIP, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-KAT3B / p300 antibody [3G230 / NM-11] - BSA and Azide free
    See all KAT3B / p300 primary antibodies
  • Description

    Mouse monoclonal [3G230 / NM-11] to KAT3B / p300 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IP, ICC/IF, ChIP, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human KAT3B/ p300.

  • Positive control

    • WB: HeLa and Jurkat whole cell lysates.
  • General notes

    ab264432 is the PBS only version of ab14984.

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    3G230 / NM-11
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Nuclear
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Rb family
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Obesity

Associated products

  • Alternative Versions

    • Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade (ab14984)
    • HRP Anti-KAT3B / p300 antibody [3G230 / NM-11] (ab200186)
  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Positive Controls

    • A431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human KAT3B / p300 protein (ab82234)

Applications

Our Abpromise guarantee covers the use of ab264432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 300 kDa (predicted molecular weight: 300 kDa).

We recommend using 3% milk as the blocking agent in Western Blot.

IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
ChIP Use 2µg for 106 cells.
IHC-P Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac). Also functions as acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Acetylates FOXO1 and enhances its transcriptional activity. Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity. Participates in CLOCK or NPAS2-regulated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter. Acetylates MTA1 at 'Lys-626' which is essential for its transcriptional coactivator activity (PubMed:10733570, PubMed:11430825, PubMed:11701890, PubMed:12402037, PubMed:12586840, PubMed:12929931, PubMed:14645221, PubMed:15186775, PubMed:15890677, PubMed:16617102, PubMed:16762839, PubMed:18722353, PubMed:18995842, PubMed:23415232, PubMed:23911289, PubMed:23934153, PubMed:8945521). Acetylates XBP1 isoform 2; acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity (PubMed:20955178). Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (PubMed:24939902). Acetylates MEF2D.
  • Involvement in disease

    Defects in EP300 may play a role in epithelial cancer.
    Chromosomal aberrations involving EP300 may be a cause of acute myeloid leukemias. Translocation t(8;22)(p11;q13) with KAT6A.
    Rubinstein-Taybi syndrome 2
  • Sequence similarities

    Contains 1 bromo domain.
    Contains 1 CBP/p300-type HAT (histone acetyltransferase) domain.
    Contains 1 KIX domain.
    Contains 2 TAZ-type zinc fingers.
    Contains 1 ZZ-type zinc finger.
  • Domain

    The CRD1 domain (cell cycle regulatory domain 1) mediates transcriptional repression of a subset of p300 responsive genes; it can be de-repressed by CDKN1A/p21WAF1 at least at some promoters. It conatins sumoylation and acetylation sites and the same lysine residues may be targeted for the respective modifications. It is proposed that deacetylation by SIRT1 allows sumoylation leading to suppressed activity.
  • Post-translational
    modifications

    Acetylated on Lys at up to 17 positions by intermolecular autocatalysis. Deacetylated in the transcriptional repression domain (CRD1) by SIRT1, preferentially at Lys-1020. Deacetylated by SIRT2, preferentially at Lys-418, Lys-423, Lys-1542, Lys-1546, Lys-1549, Lys-1699, Lys-1704 and Lys-1707.
    Citrullinated at Arg-2142 by PADI4, which impairs methylation by CARM1 and promotes interaction with NCOA2/GRIP1.
    Methylated at Arg-580 and Arg-604 in the KIX domain by CARM1, which blocks association with CREB, inhibits CREB signaling and activates apoptotic response. Also methylated at Arg-2142 by CARM1, which impairs interaction with NCOA2/GRIP1.
    Sumoylated; sumoylation in the transcriptional repression domain (CRD1) mediates transcriptional repression. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.
    Probable target of ubiquitination by FBXO3, leading to rapid proteasome-dependent degradation.
    Phosphorylated by HIPK2 in a RUNX1-dependent manner. This phosphorylation that activates EP300 happens when RUNX1 is associated with DNA and CBFB. Phosphorylated by ROCK2 and this enhances its activity. Phosphorylation at Ser-89 by AMPK reduces interaction with nuclear receptors, such as PPARG.
  • Cellular localization

    Cytoplasm. Nucleus. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus. Colocalizes with ROCK2 in the nucleus.
  • Target information above from: UniProt accession Q09472 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2033 Human
    • Entrez Gene: 170915 Rat
    • Omim: 602700 Human
    • SwissProt: Q09472 Human
    • Unigene: 517517 Human
    • Unigene: 655211 Human
    • Unigene: 12447 Rat
    • Alternative names

      • E1A associated protein p300 antibody
      • E1A binding protein p300 antibody
      • E1A-associated protein p300 antibody
      • EP300 antibody
      • EP300: E1A binding protein p300 antibody
      • EP300_HUMAN antibody
      • Histone acetyltransferase p300 antibody
      • KAT3B antibody
      • p300 HAT antibody
      • RSTS2 antibody
      see all

    Images

    • ChIP - Anti-KAT3B / p300 antibody [3G230 / NM-11] - BSA and Azide free (ab264432)
      ChIP - Anti-KAT3B / p300 antibody [3G230 / NM-11] - BSA and Azide free (ab264432)

      ChIP was performed on HeLa chromatin from 4 million cells using 8ug of ab14984. Positive control locus IRS2 shows enrichment over the negative control MyoD as expected.

      This data was provided by an anonymous collaborator.

    • Western blot - Anti-KAT3B / p300 antibody [3G230 / NM-11] - BSA and Azide free (ab264432)
      Western blot - Anti-KAT3B / p300 antibody [3G230 / NM-11] - BSA and Azide free (ab264432)
      All lanes : Anti-KAT3B / p300 antibody [3G230 / NM-11] - ChIP Grade (ab14984) at 5 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
      Lane 2 : Jurkat nuclear extract lysate (ab14844)

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

      Predicted band size: 300 kDa



      This image was produced using the same antibody clone in a different formulation ab14984.

      This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab14984 overnight at 4°C. Antibody binding was detected using an anti-mouse secondary antibody conjugated to HRP, and visualised using ECL development solution.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab264432? Please let us know so that we can cite the reference in this datasheet.

    ab264432 has not yet been referenced specifically in any publications.

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