Product nameAnti-KAT4 / TBP Associated Factor 1 antibody [EPR7145(2)]
See all KAT4 / TBP Associated Factor 1 primary antibodies
DescriptionRabbit monoclonal [EPR7145(2)] to KAT4 / TBP Associated Factor 1
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human KAT4/ TBP Associated Factor 1.
Database link: P21675
- 293T, SH-SY5Y, HepG2, Human fetal brain and Jurkat lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab168346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 280 kDa (predicted molecular weight: 213 kDa).|
FunctionLargest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle.
Involvement in diseaseDefects in TAF1 are the cause of dystonia type 3 (DYT3) [MIM:314250]; also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease.
Sequence similaritiesBelongs to the TAF1 family.
Contains 2 bromo domains.
Contains 1 HMG box DNA-binding domain.
Contains 2 protein kinase domains.
modificationsPhosphorylated by casein kinase II in vitro.
- Information by UniProt
- BA2R antibody
- CCG1 antibody
- CCGS antibody
All lanes : Anti-KAT4 / TBP Associated Factor 1 antibody [EPR7145(2)] (ab168346) at 1/1000 dilution
Lane 1 : 293T lysate
Lane 2 : SH-SY5Y lysate
Lane 3 : HepG2 lysate
Lane 4 : Human fetal brain lysate
Lane 5 : Jurkat lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 213 kDa
ab168346 has not yet been referenced specifically in any publications.