Anti-KAT4 / TBP Associated Factor 1 antibody (ab24427)


  • Product name
    Anti-KAT4 / TBP Associated Factor 1 antibody
    See all KAT4 / TBP Associated Factor 1 primary antibodies
  • Description
    Mouse polyclonal to KAT4 / TBP Associated Factor 1
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Fusion protein:


    , corresponding to amino acids 441/540 of Saccharomyces cerevisiae TAF1

  • General notes
    Produced from outbred CD1 mice

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.



Our Abpromise guarantee covers the use of ab24427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 120 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.


  • Function
    Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle.
  • Involvement in disease
    Defects in TAF1 are the cause of dystonia type 3 (DYT3) [MIM:314250]; also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease.
  • Sequence similarities
    Belongs to the TAF1 family.
    Contains 2 bromo domains.
    Contains 1 HMG box DNA-binding domain.
    Contains 2 protein kinase domains.
  • Post-translational
    Phosphorylated by casein kinase II in vitro.
  • Cellular localization
  • Information by UniProt
  • Database links
    • Alternative names
      • BA2R antibody
      • CCG1 antibody
      • CCGS antibody
      • Cell cycle G1 phase defect antibody
      • Cell cycle gene 1 protein antibody
      • Complementation of cell cycle block G1 to S antibody
      • DYT3 antibody
      • N TAF1 antibody
      • NSCL2 antibody
      • OF antibody
      • p250 antibody
      • TAF 1 antibody
      • TAF(II)250 antibody
      • TAF1 antibody
      • TAF1 RNA polymerase II TATA box binding protein (TBP) associated factor 250kDa antibody
      • TAF1_HUMAN antibody
      • TAF2A antibody
      • TAFII-250 antibody
      • TAFII250 antibody
      • TATA box binding protein (TBP) associated factor RNA polymerase II A 250kD antibody
      • TBP associated factor 250 kDa antibody
      • TBP-associated factor 250 kDa antibody
      • Transcription factor TFIID p250 polypeptide antibody
      • Transcription initiation factor TFIID 250 kDa subunit antibody
      • Transcription initiation factor TFIID subunit 1 antibody
      • XDP antibody
      see all


    • All lanes : Anti-KAT4 / TBP Associated Factor 1 antibody (ab24427) at 1/1000 dilution

      Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
      Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

      All lanes : Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

      Predicted band size: 120 kDa

      The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.


    ab24427 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Saccharomyces cerevisiae Cell lysate - whole cell (no case)
    no case
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 60 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% formaldehyde final concentr
    Detection step
    Real-time PCR
    Positive control
    The given gene in Active conditions and a very strong constitutive gene TEF1.
    Negative control
    The given gene at INACTIVE conditions and a 3" UTR of the same gene at ACTIVE conditions

    Abcam user community

    Verified customer

    Submitted Nov 22 2007


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