Recombinant
RabMAb

Anti-KAT4 / TBP Associated Factor 1 antibody [EPR7145(2)] (ab168346)

Overview

  • Product name
    Anti-KAT4 / TBP Associated Factor 1 antibody [EPR7145(2)]
    See all KAT4 / TBP Associated Factor 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR7145(2)] to KAT4 / TBP Associated Factor 1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human KAT4/ TBP Associated Factor 1.
    Database link: P21675

  • Positive control
    • 293T, SH-SY5Y, HepG2, Human fetal brain and Jurkat lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab168346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 280 kDa (predicted molecular weight: 213 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      Largest component and core scaffold of the TFIID basal transcription factor complex. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle.
    • Involvement in disease
      Defects in TAF1 are the cause of dystonia type 3 (DYT3) [MIM:314250]; also called X-linked dystonia-parkinsonism (XDP). DYT3 is a X-linked dystonia-parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT3 is characterized by severe progressive torsion dystonia followed by parkinsonism. Its prevalence is high in the Philippines. DYT3 has a well-defined pathology of extensive neuronal loss and mosaic gliosis in the striatum (caudate nucleus and putamen) which appears to resemble that in Huntington disease.
    • Sequence similarities
      Belongs to the TAF1 family.
      Contains 2 bromo domains.
      Contains 1 HMG box DNA-binding domain.
      Contains 2 protein kinase domains.
    • Post-translational
      modifications
      Phosphorylated by casein kinase II in vitro.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • BA2R antibody
      • CCG1 antibody
      • CCGS antibody
      • Cell cycle G1 phase defect antibody
      • Cell cycle gene 1 protein antibody
      • Complementation of cell cycle block G1 to S antibody
      • DYT3 antibody
      • N TAF1 antibody
      • NSCL2 antibody
      • OF antibody
      • p250 antibody
      • TAF 1 antibody
      • TAF(II)250 antibody
      • TAF1 antibody
      • TAF1 RNA polymerase II TATA box binding protein (TBP) associated factor 250kDa antibody
      • TAF1_HUMAN antibody
      • TAF2A antibody
      • TAFII-250 antibody
      • TAFII250 antibody
      • TATA box binding protein (TBP) associated factor RNA polymerase II A 250kD antibody
      • TBP associated factor 250 kDa antibody
      • TBP-associated factor 250 kDa antibody
      • Transcription factor TFIID p250 polypeptide antibody
      • Transcription initiation factor TFIID 250 kDa subunit antibody
      • Transcription initiation factor TFIID subunit 1 antibody
      • XDP antibody
      see all

    Images

    • All lanes : Anti-KAT4 / TBP Associated Factor 1 antibody [EPR7145(2)] (ab168346) at 1/1000 dilution

      Lane 1 : 293T lysate
      Lane 2 : SH-SY5Y lysate
      Lane 3 : HepG2 lysate
      Lane 4 : Human fetal brain lysate
      Lane 5 : Jurkat lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 213 kDa

    References

    ab168346 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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