Product nameAnti-KAT5 / Tip60 antibody
See all KAT5 / Tip60 primary antibodies
DescriptionRabbit polyclonal to KAT5 / Tip60
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus tropicalis
Recombinant fragment, corresponding to a region within amino acids 230-492 of Human KAT5/ Tip60.
- KAT5/Tip60 transfected 293T lysate, HeLa cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% CMIT/MIT 3:1
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137518 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 58 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionCatalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation.
Sequence similaritiesBelongs to the MYST (SAS/MOZ) family.
Contains 1 C2HC-type zinc finger.
modificationsSumoylated by UBE2I at Lys-430 and Lys-451, leading to increase of its histone acetyltransferase activity in UV-induced DNA damage response, as well as its translocation to nuclear bodies.
Phosphorylated on Ser-86 and Ser-90; enhanced during G2/M phase. Phosphorylated form has a higher activity.
Ubiquitinated by MDM2, leading to its proteasome-dependent degradation.
Cellular localizationNucleus > nucleolus. Cytoplasm > perinuclear region. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region and UV irradiation induces translocation into punctuate subnuclear structures named nuclear bodies.
- Information by UniProt
- 60 kDa Tat interactive protein antibody
- 60 kDa Tat-interactive protein antibody
- cPLA(2) interacting protein antibody
All lanes : Anti-KAT5 / Tip60 antibody (ab137518) at 1/1000 dilution
Lane 1 : Non-transfected 293T lysate
Lane 2 : KAT5 /
Tip60 transfected 293T lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 58 kDa
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labelling KAT5 / Tip60 with ab137518 at 1/200. Lower panel merged with DNA probe.
ab137518 has not yet been referenced specifically in any publications.