Key features and details
- Rabbit polyclonal to KAT5 / Tip60
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-KAT5 / Tip60 antibody
See all KAT5 / Tip60 primary antibodies
DescriptionRabbit polyclonal to KAT5 / Tip60
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
Recombinant fragment corresponding to Human KAT5/ Tip60 aa 61-292.
Database link: Q92993
- WB: Jurkat cell lysate; IHC-P: Hela xenograft tissue. Mouse duodenum tissue. Rat testis tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab151432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 58 kDa.|
|IHC-P||1/100 - 1/1000.|
FunctionCatalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation.
Sequence similaritiesBelongs to the MYST (SAS/MOZ) family.
Contains 1 C2HC-type zinc finger.
modificationsSumoylated by UBE2I at Lys-430 and Lys-451, leading to increase of its histone acetyltransferase activity in UV-induced DNA damage response, as well as its translocation to nuclear bodies.
Phosphorylated on Ser-86 and Ser-90; enhanced during G2/M phase. Phosphorylated form has a higher activity.
Ubiquitinated by MDM2, leading to its proteasome-dependent degradation.
Cellular localizationNucleus > nucleolus. Cytoplasm > perinuclear region. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region and UV irradiation induces translocation into punctuate subnuclear structures named nuclear bodies.
- Information by UniProt
- 60 kDa Tat interactive protein antibody
- 60 kDa Tat-interactive protein antibody
- cPLA(2) interacting protein antibody
All lanes : Anti-KAT5 / Tip60 antibody (ab151432) at 1/1000 dilution
Lane 1 : Jurkat whole cell lysate
Lane 2 : Jurkat nuclear lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 58 kDa
7.5% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Hela xenograft tissue labeling KAT5 / Tip60 with ab151432 at 1/500 dilution.
Paraffin embedded mouse duodenum tissue stained for TIP60 using ab151432 at 1/1000 dilution in immunohistochemical analysis.
Paraffin embedded HeLa xenograft tissue stained for TIP60 using ab151432 at 1/500 dilution in immunohistochemical analysis.
ab151432 has been referenced in 3 publications.
- Wei Y et al. Activation of KRas-ERK1/2 signaling drives the initiation and progression of glioma by suppressing the acetylation of histone H4 at lysine 16. Life Sci 225:55-63 (2019). PubMed: 30946839
- Brauns-Schubert P et al. CDK9-mediated phosphorylation controls the interaction of TIP60 with the transcriptional machinery. EMBO Rep 19:244-256 (2018). PubMed: 29335245
- Lee JH et al. EGFR-Phosphorylated Platelet Isoform of Phosphofructokinase 1 Promotes PI3K Activation. Mol Cell 70:197-210.e7 (2018). PubMed: 29677490