Overview

  • Product name

    Anti-KAT5 / Tip60 antibody
    See all KAT5 / Tip60 primary antibodies
  • Description

    Rabbit polyclonal to KAT5 / Tip60
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 61-292 of Human KAT5/ Tip60.

  • Positive control

    • Jurkat cell lysate; Hela xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab151432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 58 kDa.
IHC-P 1/100 - 1/1000.

Target

  • Function

    Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation.
  • Sequence similarities

    Belongs to the MYST (SAS/MOZ) family.
    Contains 1 C2HC-type zinc finger.
  • Post-translational
    modifications

    Sumoylated by UBE2I at Lys-430 and Lys-451, leading to increase of its histone acetyltransferase activity in UV-induced DNA damage response, as well as its translocation to nuclear bodies.
    Phosphorylated on Ser-86 and Ser-90; enhanced during G2/M phase. Phosphorylated form has a higher activity.
    Ubiquitinated by MDM2, leading to its proteasome-dependent degradation.
  • Cellular localization

    Nucleus > nucleolus. Cytoplasm > perinuclear region. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region and UV irradiation induces translocation into punctuate subnuclear structures named nuclear bodies.
  • Information by UniProt
  • Database links

  • Alternative names

    • 60 kDa Tat interactive protein antibody
    • 60 kDa Tat-interactive protein antibody
    • cPLA(2) interacting protein antibody
    • cPLA(2)-interacting protein antibody
    • cPLA2 antibody
    • cPLA2 interacting protein antibody
    • ESA1 antibody
    • Histone acetyltransferase HTATIP antibody
    • Histone acetyltransferase KAT5 antibody
    • HIV 1 Tat interactive protein antibody
    • HIV 1 Tat interactive protein, 60kDa antibody
    • HIV-1 Tat interactive protein antibody
    • HTATIP antibody
    • HTATIP1 antibody
    • K(lysine) acetyltransferase 5 antibody
    • K-acetyltransferase 5 antibody
    • KAT5 antibody
    • KAT5_HUMAN antibody
    • Lysine acetyltransferase 5 antibody
    • PLIP antibody
    • Tat interacting protein, 60kDa antibody
    • TIP antibody
    • Tip60 antibody
    see all

Images

  • All lanes : Anti-KAT5 / Tip60 antibody (ab151432) at 1/1000 dilution

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : Jurkat nuclear lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 58 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Hela xenograft tissue labeling KAT5 / Tip60 with ab151432 at 1/500 dilution.

References

This product has been referenced in:

  • Wei Y  et al. Activation of KRas-ERK1/2 signaling drives the initiation and progression of glioma by suppressing the acetylation of histone H4 at lysine 16. Life Sci 225:55-63 (2019). Read more (PubMed: 30946839) »
See 1 Publication for this product

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