Anti-KAT5 / Tip60 antibody (ab172415)
Key features and details
- Mouse polyclonal to KAT5 / Tip60
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-KAT5 / Tip60 antibody
See all KAT5 / Tip60 primary antibodies -
Description
Mouse polyclonal to KAT5 / Tip60 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Orangutan -
Immunogen
Recombinant full length protein within Human KAT5/ Tip60 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q92993 -
Positive control
- KAT5 / Tip60 transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 59 kDa. |
Target
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Function
Catalytic subunit of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome-DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Directly acetylates and activates ATM. In case of HIV-1 infection, interaction with the viral Tat protein leads to KAT5 polyubiquitination and targets it to degradation. -
Sequence similarities
Belongs to the MYST (SAS/MOZ) family.
Contains 1 C2HC-type zinc finger. -
Post-translational
modificationsSumoylated by UBE2I at Lys-430 and Lys-451, leading to increase of its histone acetyltransferase activity in UV-induced DNA damage response, as well as its translocation to nuclear bodies.
Phosphorylated on Ser-86 and Ser-90; enhanced during G2/M phase. Phosphorylated form has a higher activity.
Ubiquitinated by MDM2, leading to its proteasome-dependent degradation. -
Cellular localization
Nucleus > nucleolus. Cytoplasm > perinuclear region. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region and UV irradiation induces translocation into punctuate subnuclear structures named nuclear bodies. - Information by UniProt
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Database links
- Entrez Gene: 10524 Human
- Entrez Gene: 81601 Mouse
- Entrez Gene: 100174410 Orangutan
- Entrez Gene: 192218 Rat
- Omim: 601409 Human
- SwissProt: Q92993 Human
- SwissProt: Q8CHK4 Mouse
- SwissProt: Q5RBG4 Orangutan
see all -
Alternative names
- 60 kDa Tat interactive protein antibody
- 60 kDa Tat-interactive protein antibody
- cPLA(2) interacting protein antibody
see all
Images
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All lanes : Anti-KAT5 / Tip60 antibody (ab172415) at 1 µg/ml
Lane 1 : KAT5 / Tip60 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
Predicted band size: 59 kDa
Datasheets and documents
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Datasheet download
References (1)
ab172415 has been referenced in 1 publication.
- Wang Y et al. Transcriptional Repression of Ferritin Light Chain Increases Ferroptosis Sensitivity in Lung Adenocarcinoma. Front Cell Dev Biol 9:719187 (2021). PubMed: 34765600